Recombinant Mouse Thyrotropin receptor (Tshr)-VLPs

中文名稱：

Recombinant Mouse Thyrotropin receptor (Tshr)-VLPs
貨號(hào)：

CSB-MP025131MO(A4)
規(guī)格：

￥5000
圖片：
The purity of VLPs was greater than 95% as determined by SEC-HPLC
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 95% as determined by SEC-HPLC.
生物活性：

Not Test
基因名：

Tshr
Uniprot No.：

P47750
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

Full Length of Mature Protein
來(lái)源：

Mammalian cell
分子量：

85.7 kDa
表達(dá)區(qū)域：

22-764aa
氨基酸序列

KECASPPCECHQEDDFRVTCKELHRIPSLPPSTQTLKLIETHLKTIPSLAFSSLPNISRIYLSIDATLQRLEPHSFYNLSKMTHIEIRNTRSLTYIDPDALTELPLLKFLGIFNTGLRIFPDLTKIYSTDIFFILEITDNPYMTSVPENAFQGLCNETLTLKLYNNGFTSVQGHAFNGTKLDAVYLNKNKYLTAIDNDAFGGVYSGPTLLDVSSTSVTALPSKGLEHLKELIAKDTWTLKKLPLSLSFLHLTRADLSYPSHCCAFKNQKKIRGILESLMCNESSIRNLRQRKSVNILRGPIYQEYEEDPGDNSVGYKQNSKFQESPSNSHYYVFFEEQEDEVVGFGQELKNPQEETLQAFESHYDYTVCGDNEDMVCTPKSDEFNPCEDIMGYRFLRIVVWFVSLLALLGNIFVLLILLTSHYKLTVPRFLMCNLAFADFCMGVYLLLIASVDLYTHSEYYNHAIDWQTGPGCNTAGFFTVFASELSVYTLTVITLERWYAITFAMRLDRKIRLRHAYTIMAGGWVSCFLLALLPMVGISSYAKVSICLPMDTDTPLALAYIVLVLLLNVVAFVVVCSCYVKIYITVRNPQYNPRDKDTKIAKRMAVLIFTDFMCMAPISFYALSALMNKPLITVTNSKILLVLFYPLNSCANPFLYAIFTKAFQRDVFILLSKFGICKRQAQAYQGQRVCPNNSTGIQIQKIPQDTRQSLPNMQDTYELLGNSQLAPKLQGQISEEYKQTAL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

C-terminal 10xHis-tagged
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80℃. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項(xiàng)：

The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20℃/-80℃ upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin. Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Plays a central role in controlling thyroid cell metabolism.
基因功能參考文獻(xiàn)：
1. Data suggest that stimulation of Tsh/Tshr signaling increases apoptosis and suppresses autophagy in primary chondrocytes. PMID: 28626114
2. Unlike its critical role in maintaining the normal growth and function of the thyroid gland, our results demonstrated that hepatic TSHR is involved in liver lipid metabolism and has little effect on energy metabolism. PMID: 29421660
3. Data indicate that splenic T cells from thyroid stimulating hormone receptor (TSHR) A-subunit primed animals undergoing Graves' orbitopathy (GO) showed proliferative responses to purified TSHR antigen and secreted proinflammatory cytokines. PMID: 26872090
4. Lung fibroblasts of TSHR KO mice have decreased cell surface Igf1r expression, and have Igf1r protein and protein fragments in both cytoplasm and nucleus. Igf1r mRNA levels were similar between TSHR KO and WT mice. PMID: 26389690
5. The mechanism, underlying TSH-induced liver triglyceride accumulation, involved that TSH, through its receptor TSHR, triggered hepatic SREBP-1c activity. PMID: 25016220
6. These findings suggest that activation of TSHR directly inhibits FASN expression in mature adipocytes, possibly mediated by PKA and ERK PMID: 25655684
7. A novel role for TSHR in behavioral and neurological phenotypes of Attention deficit/hyperactivity disorder. PMID: 25016105
8. The data indicate functional TSHR is expressed in ventricular myocytes and mediates TSH-induced BNP secretion and HMGCR up-regulation through the cAMP/PKA/pCREB signaling pathway. PMID: 24065308
9. findings demonstrate TSH-R expression is thymus-specific within the immune system; data support the notion of a novel neuroendocrine-immune interaction in which TSH-R signaling in the thymus, most likely mediated by TSH, enhances thymic T-cell development PMID: 24635198
10. The inhibition of hepatic PEPCK and G6P and enhanced expression of GK contributed to the development of fasting hypoglycemia in Tshr-ko mice. PMID: 23665701
11. UCP-1 and TSHR co-localized in retrobulbar adipose tissues in murine models of Graves' disease. PMID: 23386414
12. Cloned a thyroid-stimulating hormone receptor (TSHR) cDNA from thyroid glands. The sequence of this cDNA indicated that it encoded a 739 amino acid TSHR splice variant that lacked exon 5 (TSHR739). PMID: 23538203
13. Study with mouse monoclonal antibody 3BD10 confirms TSHR conformational heterogeneity and suggests that the N-terminal cysteine cluster may contribute to this structural variability. PMID: 23183178
14. Messenger RNA levels of hormone-sensitive lipase in epididymal fat containing the transferred TSHR gene were twofold higher than those in tissue from the control side PMID: 22496347
15. Data demonstrate the key role of TSH signaling in Braf-induced papillary thyroid cancer initiation and provide experimental support for observations in humans pointing to a strong association between TSH levels and thyroid cancer incidence. PMID: 21220306
16. Studies indicate it is important to refine knowledge and insights in non-autoimmune diseases caused by defects of the TSHR gene and it might help to develop pharmacological means for compensation of uncontrolled thyroid growth. PMID: 20515734
17. TSHR KO mice are, therefore, of value for future studies dissecting the autoimmune response to the mouse TSHR. PMID: 20555026
18. adipocyte TSHr under normal conditions affects adipocyte growth and development. PMID: 20152797
19. Consequences of hypothyroidism on auditory system function in Tshr mutant (hyt) mice. PMID: 11833606
20. Development of auditory brainstem responses (ABRs) in Tshr mutant mice derived from euthyroid and hypothyroid dams. PMID: 11833607
21. Knockout mice were used to study the role of the TSHR signaling in thyroid development & differentiation. The major role of the TSH/TSHR pathway is controlling genes involved in iodide metabolism. PMID: 12432093
22. TSHR expression was required for expression of sodium-iodide symporter but was not required for thyroglobulin expression, suggesting that the thyroid hormone synthetic pathway of the mouse could be dissociated into TSHR-dependent and -independent steps. PMID: 12432094
23. TSHR action is complemented by RII-PKAII which stably propagates robust cAMP signals in cell compartments PMID: 12902333
24. Ttf1 haploinsufficiency causes a reduction in the expression of TSHr and thyroglobulin, genes with TTF1 binding sites in their promoter regions PMID: 12907760
25. Pituitary folliculo-stellate cells express functional TSHR not coupled to cAMP nor IP3 but probably signals through JAK/STAT5a pathway. In vitro model to study role of TSHR in pituitary folliculo-stellate cells PMID: 15662590
26. Higher somatic mutations within complementarity-determining region 3 may correlate with pathogenic antibodies against TSH receptor. PMID: 17068139
27. Tshr(-/-) mice produced normal numbers of cones, indicating that the major requirement for thyroid hormone is in opsin patterning rather than in cone generation. PMID: 18974269
28. Melatonin activates Tshr expression in the pars tuberalis but not in the ependymal cell layer of the infundibular recess. PMID: 19589858
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相關(guān)疾病：

Defects in Tshr are the cause of hyt/hyt hypothyroidism, an autosomal recessive, fetal-onset, severe hypothyroidism related to TSH hyporesponsiveness and associated with elevated TSH.
亞細(xì)胞定位：

Cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein.
蛋白家族：

G-protein coupled receptor 1 family, FSH/LSH/TSH subfamily
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:22095

STRING: 10090.ENSMUSP00000021346

UniGene: Mm.173847