Recombinant Human Proteinase-activated receptor 3 (F2RL2)

中文名稱：

人F2RL2重組蛋白
貨號：

CSB-CF007926HU
規(guī)格：

￥9720
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

F2RL2
Uniprot No.：

O00254
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
來源：

in vitro E.coli expression system
分子量：

40.0 kDa
表達區(qū)域：

39-374aa
氨基酸序列

TFRGAPPNSFEEFPFSALEGWTGATITVKIKCPEESASHLHVKNATMGYLTSSLSTKLIPAIYLLVFVVGVPANAVTLWMLFFRTRSICTTVFYTNLAIADFLFCVTLPFKIAYHLNGNNWVFGEVLCRATTVIFYGNMYCSILLLACISINRYLAIVHPFTYRGLPKHTYALVTCGLVWATVFLYMLPFFILKQEYYLVQPDITTCHDVHNTCESSSPFQLYYFISLAFFGFLIPFVLIIYCYAAIIRTLNAYDHRWLWYVKASLLILVIFTICFAPSNIILIIHHANYYYNNTDGLYFIYLIALCLGSLNSCLDPFLYFLMSKTRNHSTAYLTK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis.
基因功能參考文獻：
1. PAR3 is essential in thrombin stimulated insulin secretion. PMID: 26742564
2. TFRGAP, a PAR-3 mimicking peptide significantly induced the phosphorylation of eNOS-Thr-495 with minimal phosphorylation of eNOS-Ser-1177 with no change in nitric oxide production. PMID: 26729042
3. These data provide intriguing novel insights into the diversification of functional selectivity of protease signaling achievable by canonical and noncanonical PAR activation, such as the activation of vascular-protective Tie2 by noncanonical PAR3 activation. PMID: 25320242
4. In pancreatic adenocarcinoma cells, PAR3 knockdown enhances cell adhesion. We propose this is due to increased expression of E-cadherin, leading to a greater adhesion of free-floating cells to cells bound to the surface via integrins, particularly ITGalphav. PMID: 24699825
5. Elevated expression of PAR-3, but not PAR-4, was detected in the lungs of idiopathic pulmonary fibrosis patients. PMID: 23739922
6. A unique contributory role for PAR3 in the complex mechanisms underlying Activated protein C cytoprotective effects. PMID: 23788139
7. PAR-3 receptors interact with thrombin and increase heme oxygenase-1 expression in synovial fibroblasts. PMID: 22541814
8. Human PAR-3 is regulated post-transcriptionally via the mRNA-stabilizing factor HuR, whereas transcriptional control involves nuclear factor of activated T cells (NFAT). PMID: 21596928
9. FXa bound in a punctate manner to thrombi under shear, while thrombin and fibrin(ogen) distributed ubiquitously over platelet-fibrin thrombi PMID: 20454680
10. genetic polymorphism is associated with peripheral arterial disease severity PMID: 20390230
11. A novel role is identified for PAR3 in thrombin signaling. PMID: 20442298
12. Down-regulation of connexin and par-3 signals early anaplasia and malignant change in rectal cancer. PMID: 19513616
13. upregulated thrombin generation and inhibition of fibrinolysis, occurred in one-third of the Stem cell transplant patients associating with the development of Graft versus host disease PMID: 19718071
14. Lower plasma levels of thrombin-antithrombin complex correlate to higher recanalisation rates after ischaemic stroke PMID: 19806263
15. PAR-3 is expressed in human SMC and triggers intracellular calcium signaling. PMID: 14515192
16. expression of PAR3 mRNA and protein in cells undergoing megakaryocytic maturation suggests a developmental role for PAR3; regulation of PAR3 expression appears to be specifically coupled to the protein kinase C system. PMID: 15504554
17. a Na-independent Cl-/HCO3- exchange mechanism mediates the recovery of pHi from alkalosis in platelets and that thrombin activates this exchanger by a direct regulatory pathway PMID: 15850610
18. The changes in levels of blood coagulation factor ii in folic acid deficient rats are reversed by treatment with folic acid. PMID: 16046705
19. F2R genetic variants may influence the natural history of CHD in patients at high risk of cardiovascular events. PMID: 17347481
20. PAR-3 is able to signal autonomously to induce IL-8 release mediated by ERK1/2 phosphorylation, which contributes actively to inflammatory responses PMID: 18264801
21. Thrombin-mediated increases in cytosolic [Ca2+] involve different mechanisms in human pulmonary artery smooth muscle and endothelial cells. PMID: 18836030
22. This study demonstrates that the anticoagulant profile of thrombin often caused by a mutation of the thrombin scaffold finds its likely molecular origin in the stabilization of the inactive E* form. PMID: 19473969
23. Mechanism of the anticoagulant activity of thrombin mutant W215A/E217A. PMID: 19586901
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亞細胞定位：

Cell membrane; Multi-pass membrane protein.
蛋白家族：

G-protein coupled receptor 1 family
組織特異性：

Highest expression in the megakaryocytes of the bone marrow, lower in mature megakaryocytes, in platelets and in a variety of other tissues such as heart and gut.
數(shù)據(jù)庫鏈接：

HGNC: 3539

OMIM: 601919

KEGG: hsa:2151

STRING: 9606.ENSP00000296641

UniGene: Hs.42502