Recombinant Human H (+)/Cl (-)exchange transporter 3 (CLCN3)-VLPs

中文名稱：

Recombinant Human H(+)/Cl(-) exchange transporter 3(CLCN3)
貨號：

CSB-MP005482HU(A4)
規格：
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

基因名：

CLCN3
Uniprot No.：

P51790
別名：

CLCN3; H(+/Cl(- exchange transporter 3; Chloride channel protein 3; ClC-3; Chloride transporter ClC-3
種屬：

Homo sapiens (Human)
來源：

Mammalian cell
表達區域：

1-818aa
氨基酸序列

MASMTGGQQMGRDPMESEQLFHRGYYRNSYNSITSASSDEELLDGAGVIMDFQTSEDDNLLDGDTAVGTHYTMTNGGSINSSTHLLDLLDEPIPGVGTYDDFHTIDWVREKCKDRERHRRINSKKKESAWEMTKSLYDAWSGWLVVTLTGLASGALAGLIDIAADWMTDLKEGICLSALWYNHEQCCWGSNETTFEERDKCPQWKTWAELIIGQAEGPGSYIMNYIMYIFWALSFAFLAVSLVKVFAPYACGSGIPEIKTILSGFIIRGYLGKWTLMIKTITLVLAVASGLSLGKEGPLVHVACCCGNIFSYLFPKYSTNEAKKREVLSAASAAGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWRSFFAALVAAFVLRSINPFGNSRLVLFYVEYHTPWYLFELFPFILLGVFGGLWGAFFIRANIAWCRRRKSTKFGKYPVLEVIIVAAITAVIAFPNPYTRLNTSELIKELFTDCGPLESSSLCDYRNDMNASKIVDDIPDRPAGIGVYSAIWQLCLALIFKIIMTVFTFGIKVPSGLFIPSMAIGAIAGRIVGIAVEQLAYYHHDWFIFKEWCEVGADCITPGLYAMVGAAACLGGVTRMTVSLVVIVFELTGGLEYIVPLMAAVMTSKWVGDAFGREGIYEAHIRLNGYPFLDAKEEFTHTTLAADVMRPRRNDPPLAVLTQDNMTVDDIENMINETSYNGFPVIMSKESQRLVGFALRRDLTIAIESARKKQEGIVGSSRVCFAQHTPSLPAESPRPLKLRSILDMSPFTVTDHTPMEIVVDIFRKLGLRQCLVTHNGRLLGIITKKDILRHMAQTANQDPASIMFN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 10xHis-tagged
If you have specified tag type, please tell us and we will check if it’s possible to develop.
產品提供形式：

Lyophilized powder
Note: We will default ship it in lyophilized form with normal bule ice packs. However, if you request to ship in liquid form, it needs to be shipped with dry ice, please communicate with us in advance and extra fees for dry ice and dry ice box will be charged.
緩沖液：

Lyophilized from PBS, 6% Trehalose, pH 7.4
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store the protein at -20°C/-80°C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons. The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The presence of conserved gating glutamate residues is typical for family members that function as antiporters.; Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons.
基因功能參考文獻：
1. Unique oligomerization properties of ClC-4 permit regulated targeting of ClC-4 to various endosomal compartment systems via expression of different ClC-3 splice variants. PMID: 28972156
2. we demonstrated that ClC-3 can arrest the cell cycle at the G1 phase to inhibit cell proliferation, suggesting that ClC-3 has the potential to be a novel target for hepatocellular carcinoma (HCC) therapy and potentially improve the prognosis of HCC patients PMID: 29749557
3. CLC-3 may get involved in proliferation, invasion, and migration of ovarian cancer cells and thus may be a useful therapeutic target. PMID: 29787012
4. ClC-3 is a potential target of 17beta-estradiol and is modulated by the ERalpha in breast cancer cells. PMID: 28419445
5. Study provided novel and compelling evidence for the functional role of the unique CLC-3, which are significantly upregulated during ischemia, in the protection of the heart under stress PMID: 27064645
6. ClC-3 promotes endometriotic cell migration and invasion. PMID: 26965430
7. these results demonstrated that ClC-3 is involved in the proliferation and migration of osteosarcoma cell PMID: 25973047
8. Data indicate that cytoplasmic chloride channel-3 (ClC-3) plays an active and key role in tumor metastasis and may be a valuable prognostic biomarker and a therapeutic target to prevent tumor spread. PMID: 25537517
9. CLC3 is required in the activation and migration of human blood eosinophils. PMID: 25514499
10. Authors summarize the function of CLC-3 in cancer and discuss the mechanisms by which CLC-3 contributes to proliferation, apoptosis and drug resistance in cancer cells. [Review] PMID: 25421907
11. swelling-activated Cl currents and CLC-3 play a role in pulmonary artery smooth muscle cell proliferation, but CLC-3 channels do not underlie swelling in these cells PMID: 24284495
12. ClC-3 deficiency inhibited Ang II-induced EPC apoptosis via suppressing ROS generation derived from NADPH oxidase. PMID: 23873092
13. Suggest that ClC-3 suppression causes the inhibition of Akt and autophagy, which can enhance the therapeutic benefit of cisplatin in U251 cells. PMID: 23408563
14. K(Ca)3.1 and ClC-3 are expressed in tissue samples obtained from patients diagnosed with grade IV gliomas. Both K(Ca)3.1 and ClC-3 colocalize to the invading processes of glioma cells PMID: 23345219
15. Data indicate in umbilical vein endothelial cells transfected with ClC-3 siRNA showed activation of NF-kappaB. PMID: 23006728
16. results reveal a cell cycle-dependent change of the subcellular distribution of ClC-3 and strongly suggest that ClC-3 has nucleocytoplasmic shuttling dynamics that may play key regulatory roles during different stages of the cell cycle in tumor cells. PMID: 22371056
17. ClC-3 is a candidate of the channel proteins that mediate or regulate the acid-activated chloride current in nasopharyngeal carcinoma cells. PMID: 22496242
18. ClC-3 protein may be considered as a potential tumor marker and therapeutic target for human nasopharyngeal carcinoma. PMID: 22108225
19. ClC-3 may be a main component of background chloride channels which can be activated under isotonic conditions by autocrine/paracrine ATP through purinergic receptor pathways; the background current is involved in maintenance of basal cell volume. PMID: 21792908
20. ClC-3 is involved in the activation of volume-activated chloride currents but not of stretch-activated chloride currents in hepatocellular carcinoma cells. PMID: 20945353
21. High ClC-3 is associated with extensive migration and invasion in glioma. PMID: 21115901
22. ClC-3 is an important molecular component underlying VSOACs and the RVD process in HeLa cells PMID: 12183454
23. results show that the ClC-3B PDZ-binding isoform resides in the Golgi where it co-localizes with a small amount of CFTR (cystic fibrosis transmembrane conductance regulator) PMID: 12471024
24. results suggest a fundamental role of endogenous ClC-3 in the swelling-activated Cl- channels function and cell volume regulation in human gastric epithelial cells PMID: 12842831
25. ClC-3 and ZnT3 reside in a common vesicle population where they functionally interact to determine vesicle luminal composition. PMID: 15073168
26. volume-regulated ClC-3 Cl(-) channels play important role in the regulation of [Cl(-)](i) and cell proliferation of vascular smooth muscle cells PMID: 15596438
27. Clcn3 was considered the most likely candidate of Cl- channel involved in volume regulation of human sperm. PMID: 16033995
28. ClC-3 is required for normal neutrophil oxidative function, phagocytosis, and transendothelial migration PMID: 16522634
29. This study demonstrates that superoxide flux across the endothelial cell plasma membrane occurs through chloride channel-3 channels and induces intracellular calcium release, which activates mitochondrial superoxide generation. PMID: 17360969
30. The relative density of CLC-3 mRNA was 0.22+/-0.09 and 0.12+/-0.05 in HNECs treated with 3 and 0.9% saline, respectively. PMID: 17869465
31. CLC-3 is upregulated in ethmoid mucosa and may affect the development of chronic rhinosinusitis without nasal polyps. PMID: 17882904
32. inhibition of the NADPH oxidase or ClC-3 in otherwise unstimulated cells elicited a phenotype similar to that seen after endotoxin priming, suggesting that basal oxidant production helps to maintain cellular quiescence. PMID: 17908687
33. oxidation induces surface expression of ClC-3 and activation of a ClC-3-dependent anion permeability in K562 cells PMID: 17976378
34. ClC-3 overexpression induced a pH-sensitive conductance in HEK293T cells that is biophysically similar to ClC-4 and ClC-5. PMID: 17977943
35. These data confirm that ClC-3 is important in VRAC function and cell volume regulation, is associated with the I(Cl,LPA) current activity, and participates in the fibroblast-to-myofibroblast transition. PMID: 18077605
36. An essential role of sClC-3 in native volume-sensitive outwardly rectifying anion channels function in mouse atrial myocytes. PMID: 18986326
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亞細胞定位：

[Isoform 1]: Early endosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.; [Isoform 2]: Golgi apparatus membrane; Multi-pass membrane protein. Cell projection, ruffle membrane; Multi-pass membrane protein.
蛋白家族：

Chloride channel (TC 2.A.49) family, ClC-3/CLCN3 subfamily
組織特異性：

Expressed primarily in tissues derived from neuroectoderm. Within the brain, its expression is particularly evident in the hippocampus, olfactory cortex, and olfactory bulb. Highly expressed in aortic and coronary vascular smooth muscle cells, and aortic
數據庫鏈接：

HGNC: 2021

OMIM: 600580

KEGG: hsa:1182

STRING: 9606.ENSP00000261514

UniGene: Hs.481186