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Recombinant Drosophila melanogaster Protein white (w)

  • 中文名稱:
    黑腹果蠅w重組蛋白
  • 貨號:
    CSB-CF320613DLU
  • 規格:
  • 來源:
    in vitro E.coli expression system
  • 其他:

產品詳情

  • 基因名:
    w
  • Uniprot No.:
  • 別名:
    w; CG2759; Protein white
  • 種屬:
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-687
  • 氨基酸序列
    MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNYGTLLPPSPPEDSGSG SGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERHIPAPRKHLLKNVCGVA YPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNGQPVDAKEMQARCAYVQQ DDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQELSLSKCQHTIIGVPGRVKG LSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQVLKKLSQKGKTVILTIHQP SSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCPTNYNPADFYVQVLAVVPGRE IESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQPENGYTYKATWFMQFRAVLWRS WLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVGVMNINGAIFLFLTNMTFQNVFA TINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPLFLTVPLVFTAIAYPMIGLRAGVL HFFNCLALVTLVANVSTSFGYLISCASSSTSMALSVGPPVIIPFLLFGGFFLNSGSVPVY LKWLSYLSWFRYANEGLLINQWADVEPGEISCTSSNTTCPSSGKVILETLNFSAADLPLD YVGLAILIVSFRVLAYLALRLRARRKE
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 10xHis-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Part of a membrane-spanning permease system necessary for the transport of pigment precursors into pigment cells responsible for eye color. White dimerize with brown for the transport of guanine. Scarlet and white complex transports a metabolic intermediate (such as 3-hydroxy kynurenine) from the cytoplasm into the pigment granules.
  • 基因功能參考文獻:
    1. Using a transgenic lines, we show that the Fab-7 insulator of the bithorax complex and the MDG4 (gypsy) insulator are able to disrupt weak transcription derived from the enhancer regulating the white gene expression in the eyes. PMID: 29372797
    2. data suggest that a White-cGMP interaction modulates the timing of locomotor recovery from anoxia PMID: 28060942
    3. Data suggest that age-dependent behavioral/physiological changes differ between wide-type flies (Canton-S flies) and genetically engineered flies null mutation of white gene (w1118 flies); old w1118 flies exhibit decreased path length per minute and reduced 0.2 s path increment compared with young flies, whereas old Canton-S flies exhibit same path length per minute and same 0.2 s path increment compared with young flies. PMID: 28087331
    4. White modulates the timing of locomotor recovery from anoxia. PMID: 27029736
    5. this study we have used the CRISPR/Cas9 system to introduce site-specific mutations in the D. suzukii white (w) and Sex lethal (Sxl) genes. PMID: 26721433
    6. Genetic analysis with a series of crosses revealed that the White gene was not associated with the phenotype of boundary preference in wildtype flies. PMID: 26351842
    7. it was observed that Mod(mdg4)-67.2, a component of the gypsy insulator, interacted with the Zeste protein, which is critical for the eye enhancer-white promoter communication. PMID: 23861668
    8. Using genetics and pharmacology, we identified the metabolite transporter White and white-dependent serotonin as suppressors of phototactic personality PMID: 23150588
    9. H3K9me2 levels at the white gene directly correlate with its level of expression. PMID: 22634714
    10. Data show that maize B1 transgenic lines exhibit cis-silencing of white gene but epigenetic activation of white gene expression in trans. PMID: 22729404
    11. This allele enhances mutations in the subset of other eye-color genes with phenotypes similar to garnet. PMID: 11962627
    12. The promoter and enhancer region of white gene was studied. PMID: 16480133
    13. An insulator element is almost contiguous to the white 3'UTR. PMID: 18086699
    14. Assay of cGMP transport in w(-) (mutant) tubules shows that w is required for cGMP transport but not cAMP transport. Targeted over-expression of w in w(-) tubule principal cells significantly increases cGMP transport compared with that in w(-) controls. PMID: 18310115

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  • 亞細胞定位:
    Membrane; Multi-pass membrane protein. Cytoplasmic granule membrane; Multi-pass membrane protein. Note=Pigment granules within pigment cells and retinula cells of the compound eye (when complexed with st).
  • 蛋白家族:
    ABC transporter superfamily, ABCG family, Eye pigment precursor importer (TC 3.A.1.204) subfamily
  • 數據庫鏈接:

    KEGG: dme:Dmel_CG2759

    STRING: 7227.FBpp0070468

    UniGene: Dm.19661



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