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Recombinant Arabidopsis thaliana Protein TIC 40, chloroplastic (TIC40)

  • 中文名稱(chēng):
    擬南芥TIC40重組蛋白
  • 貨號(hào):
    CSB-CF884298DOA
  • 規(guī)格:
  • 來(lái)源:
    in vitro E.coli expression system
  • 其他:

產(chǎn)品詳情

  • 基因名:
    TIC40
  • Uniprot No.:
  • 別名:
    TIC40; PDE120; At5g16620; MTG13.6; Protein TIC 40, chloroplastic; Protein PIGMENT DEFECTIVE EMBRYO 120; Translocon at the inner envelope membrane of chloroplasts 40; AtTIC40
  • 種屬:
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長(zhǎng)度:
    Full Length of Mature Protein
  • 表達(dá)區(qū)域:
    77-447
  • 氨基酸序列
    SIFSSSRDQQTTSVASPSVPVPPPSSSTIGSPLFWIGVGVGLSALFSYVTSNLKKYAMQT AMKTMMNQMNTQNSQFNNSGFPSGSPFPFPFPPQTSPASSPFQSQSQSSGATVDVTATKV ETPPSTKPKPTPAKDIEVDKPSVVLEASKEKKEEKNYAFEDISPEETTKESPFSNYAEVS ETNSPKETRLFEDVLQNGAGPANGATASEVFQSLGGGKGGPGLSVEALEKMMEDPTVQKM VYPYLPEEMRNPETFKWMLKNPQYRQQLQDMLNNMSGSGEWDKRMTDTLKNFDLNSPEVK QQFNQIGLTPEEVISKIMENPDVAMAFQNPRVQAALMECSENPMNIMKYQNDKEVMDVFN KISQLFPGMTG
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽:
    N-terminal 10xHis-tagged
  • 產(chǎn)品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 儲(chǔ)存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng):
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Involved in protein precursor import into chloroplasts. Part of the motor complex consisting of a co-chaperone (TIC40) and a chaperone (HSP93) associated with the import channel (TIC110). Causes the release of bound transit peptides from TIC110 and stimulates ATP hydrolysis by HSP93. Involved in reinsertion of proteins from the chloroplast stroma into the inner membrane.
  • 基因功能參考文獻(xiàn):
    1. Tic40 is a multifunctional protein that, in addition to its originally described role in protein import, is able to influence downstream processes leading to thylakoid biogenesis. PMID: 28684427
    2. Interaction of Tic40-NP and Hsp93 stimulates ATP hydrolysis of Hsp93, but the hydrolysis is abolished in both N320A and N329A mutants of Tic40-NP. PMID: 22888115
    3. The interaction of Tic110, Tic40 and Hsp93 in A. thaliana chloroplast protein transport is described. [AtTic40] PMID: 15659100
    4. The interaction of Tic40 with rhomboid proteases in a yeast mitochondria-based assay is reported. PMID: 17327256
    5. analysis of similarity between the chloroplast translocon component, Tic40, and the human co-chaperone, Hsp70-interacting protein PMID: 17535810
    6. the sorting of AtTic40 requires a bipartite transit peptide, which is first cleaved by the stromal processing peptidase (SPP), thus generating a soluble AtTic40 stromal intermediate. PMID: 17883373
    7. In a Tic40 mutant soluble Tic40 and Tic110N intermediates accumulated in the chloroplast stroma due to a slower rate of reinsertion into the inner membrane. PMID: 18657235

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  • 亞細(xì)胞定位:
    Plastid, chloroplast inner membrane; Single-pass membrane protein.
  • 組織特異性:
    Expressed in seedlings, flowers, leaves, stems and roots.
  • 數(shù)據(jù)庫(kù)鏈接:

    KEGG: ath:AT5G16620

    STRING: 3702.AT5G16620.1

    UniGene: At.25049



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