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Recombinant Arabidopsis thaliana L-galactono-1,4-lactone dehydrogenase, mitochondrial (GLDH)

  • 中文名稱:
    擬南芥GLDH重組蛋白
  • 貨號:
    CSB-CF890441DOA
  • 規格:
  • 來源:
    in vitro E.coli expression system
  • 其他:

產品詳情

  • 基因名:
    GLDH
  • Uniprot No.:
  • 別名:
    GLDH; At3g47930; T17F15.200; L-galactono-1,4-lactone dehydrogenase, mitochondrial
  • 種屬:
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度:
    Full Length of Mature Protein
  • 表達區域:
    102-610
  • 氨基酸序列
    YAPLPEDLHTVSNWSGTHEVQTRNFNQPENLADLEALVKESHEKKLRIRPVGSGLSPNGI GLSRSGMVNLALMDKVLEVDKEKKRVTVQAGIRVQQLVDAIKDYGLTLQNFASIREQQIG GIIQVGAHGTGARLPPIDEQVISMKLVTPAKGTIELSREKDPELFHLARCGLGGLGVVAE VTLQCVARHELVEHTYVSNLQEIKKNHKKLLSANKHVKYLYIPYTDTVVVVTCNPVSKWS GPPKDKPKYTTDEAVQHVRDLYRESIVKYRVQDSGKKSPDSSEPDIQELSFTELRDKLLA LDPLNDVHVAKVNQAEAEFWKKSEGYRVGWSDEILGFDCGGQQWVSESCFPAGTLANPSM KDLEYIEELKKLIEKEAIPAPAPIEQRWTARSKSPISPAFSTSEDDIFSWVGIIMYLPTA DPRQRKDITDEFFHYRHLTQKQLWDQFSAYEHWAKIEIPKDKEELEALQARIRKRFPVDA YNKARRELDPNRILSNNMVEKLFPVSTTA
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 10xHis-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Involved in the biosynthesis of ascorbic acid. Required for the accumulation of respiratory complex I. Uses L-galactono-1,4-lactone and L-gulono-1,4-lactone as substrates, but not D-galactono-1,4-lactone, D-gulono-1,4-lactone, L-mannono-1,4-lactone or D-galactonic acid. Also active with phenazine methosulfate and 1,4-benzoquinone as electron acceptors.
  • 基因功能參考文獻:
    1. GLDH is a dual-function protein that has a second, non-enzymatic function in complex I assembly as a plant-specific assembly factor. PMID: 26520835
    2. a role of GLDH during complex I formation, which is based on its binding to specific assembly intermediates. PMID: 22378782
    3. Recombinant GALDH from Arabidopsis thaliana is inactivated by hydrogen peroxide due to selective oxidation of cysteine (Cys)-340, located in the cap domain. [GALDH] PMID: 19369590
    4. Data show that AtGALDH forms a flavin N5 sulfite adduct and that AtGALDH lacks the histidine involved in covalent FAD binding, but contains a leucine instead. PMID: 18190525
    5. L-galactono-1,4-lactone dehydrogenase is required for the accumulation of respiratory complex I PMID: 18799460

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  • 亞細胞定位:
    Mitochondrion membrane; Single-pass membrane protein.
  • 數據庫鏈接:

    KEGG: ath:AT3G47930

    STRING: 3702.AT3G47930.1

    UniGene: At.1530



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