Recombinant Zea mays Thiamine thiazole synthase 1, chloroplastic (THI1-1)
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中文名稱:玉米THI1-1重組蛋白
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貨號:CSB-EP677068ZAX-B
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說明書:
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規格:
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來源:E.coli
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共軛:Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他:
產品詳情
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純度:>85% (SDS-PAGE)
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基因名:THI1-1
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Uniprot No.:
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別名:THI1-1; Thiamine thiazole synthase 1; chloroplastic; EC 2.4.2.60; Thiazole biosynthetic enzyme 1
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種屬:Zea mays (Maize)
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蛋白長度:Full Length of Mature Protein
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表達區域:46-354
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氨基酸序列ASMSM SSSNPPYDLT SFRFSPIKES IVSREMTRRY MTDMITYADT DVVIVGAGSA GLSCAYELSK DPAVSIAIVE QSVSPGGGAW LGGQLFSAMV VRKPAHLFLD ELGVAYDEAE DYVVIKHAAL FTSTVMSLLL ARPNVKLFNA VAVEDLIVRG GRVGGVVTNW ALVSMNHDTQ SCMDPNVMEA KVVVSSCGHD GPFGATGVKR LQDIGMISAV PGMKALDMNT AEDEIVRLTR EVVPGMIVTG MEVAEIDGAP RMGPTFGAMM ISGQKAAHLA LKALGRPNAV DGTMSPPLRE ELMIAYKDDE VVDA
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蛋白標簽:Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產品提供形式:Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復溶:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期:Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項:Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet :Please contact us to get it.
靶點詳情
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功能:Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.
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亞細胞定位:Plastid, chloroplast.
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蛋白家族:THI4 family
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組織特異性:Highest expression in developing embryos and green leaves and a very low level expression seen in endosperm, roots, etiolated shoots and immature ears.
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數據庫鏈接:
KEGG: zma:542714
UniGene: Zm.288
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