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  4. Recombinant Xenopus laevis Ubiquitin-like modifier-activating enzyme 5 (uba5)

Recombinant Xenopus laevis Ubiquitin-like modifier-activating enzyme 5 (uba5)

  • 中文名稱：
    有爪蟾蜍uba5重組蛋白
  • 貨號：
    CSB-YP665978XBE
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    有爪蟾蜍uba5重組蛋白
  • 貨號：
    CSB-EP665978XBE
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    有爪蟾蜍uba5重組蛋白
  • 貨號：
    CSB-EP665978XBE-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    有爪蟾蜍uba5重組蛋白
  • 貨號：
    CSB-BP665978XBE
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    有爪蟾蜍uba5重組蛋白
  • 貨號：
    CSB-MP665978XBE
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    uba5
  • Uniprot No.：
  • 別名：
    uba5; ube1dc1; Ubiquitin-like modifier-activating enzyme 5; Ubiquitin-activating enzyme 5; UFM1-activating enzyme; Ubiquitin-activating enzyme E1 domain-containing protein 1
  • 種屬：
    Xenopus laevis (African clawed frog)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-397
  • 氨基酸序列
    MEGLIEELRS RVRELEEELD RVRNGQHEGH RTKIEKMSAE VVDSNPYSRL MALKRMGIVE NYEKIRTFTV AVVGVGGVGS VTAEMLTRCG IGKLLLFDYD KVELANMNRL FFQPHQAGLS KVEAAEHTLR NINPDVQFEV HNYNITTLDN FQHFMDRISK GGLKEGSPVD LVLSCVDNFE ARMAINTACN ELGQVWMESG VSENAVSGHI QLIKPGETAC FACAPPLVVA ANIDEKTLKR EGVCAASLPT TMGVVAGILV QNVLKYLLNF GTVSFYLGYN AMQDFFPTMA MKPNPQCDDK YCRKQQEEFK LKEAAKPKQE TVVVEEEEVV HEDNDWGIEL VSEVSEEELK AASGPVPDLP EGIKVAYTIP ITKPTSGFTV EDSEQSLDEL MAQMKNL
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    E1-like enzyme which specifically catalyzes the first step in ufmylation. Activates ufm1 by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ufm1-E1 thioester and free AMP. Activates ufm1 via a trans-binding mechanism, in which ufm1 interacts with distinct sites in both subunits of the uba5 homodimer. Trans-binding also promotes stabilization of the uba5 homodimer, and enhances ATP-binding. Transfer of ufm1 from uba5 to the E2-like enzyme UFC1 also takes place using a trans mechanism. Ufmylation is involved in reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress.
  • 亞細胞定位：
    Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Golgi apparatus.
  • 蛋白家族：
    Ubiquitin-activating E1 family, UBA5 subfamily
  • 數據庫鏈接：

    KEGG: xla:734791

    UniGene: Xl.42315



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