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  4. Recombinant Xenopus laevis Polyribonucleotide 5'-hydroxyl-kinase Clp1 (clp1)

Recombinant Xenopus laevis Polyribonucleotide 5'-hydroxyl-kinase Clp1 (clp1)

  • 中文名稱：
    Recombinant Xenopus laevis Polyribonucleotide 5'-hydroxyl-kinase Clp1(clp1)
  • 貨號：
    CSB-YP740650XBE
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Xenopus laevis Polyribonucleotide 5'-hydroxyl-kinase Clp1(clp1)
  • 貨號：
    CSB-EP740650XBE-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Xenopus laevis Polyribonucleotide 5'-hydroxyl-kinase Clp1(clp1)
  • 貨號：
    CSB-BP740650XBE
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Xenopus laevis Polyribonucleotide 5'-hydroxyl-kinase Clp1(clp1)
  • 貨號：
    CSB-MP740650XBE
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    clp1
  • Uniprot No.：
  • 別名：
    clp1Polyribonucleotide 5'-hydroxyl-kinase Clp1; EC 2.7.1.78; Polyadenylation factor Clp1; Polynucleotide kinase Clp1; Pre-mRNA cleavage complex II protein Clp1
  • 種屬：
    Xenopus laevis (African clawed frog)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-439
  • 氨基酸序列
    MSSEDLSAPG PSAPDSSWSS TGPSTKFELE RETELRLEVE GTDPVRVELV SGLAEVFGTE LTRNKKYTFP PGSRAAIFTW HGCTVQLWGS PDMAYVSRDT PMLLYLNTQV GLEQMRVQAE REGERGPRVL VAGPSDVGKS TLCRLLLNYA VRRGRRPTLV ELDVGQGSVS VPGTMGALCV ERPADVEEGF SAQAPLVYHF GSTTPGTNIK LYNKLTSRLA HVFNLRCDSN RRASVSGCLI NTCGWVKGSG YQALIHAASA FEVDVVLVLD QERLYNDLLR DLPHFVRTLL LPKSGGASER SKECRRESRD QRVREYFYGP RGSLYPHAFE IKFSEVRVYK VGAPTIPDSC LPLGMSQEDN QLKLVPVTPG RDMAHHLLSV VPLDGGSAEE GIEERSVAGF IVITGVDTER QTLTLLSPAP RPLPKCVLLI MDIRFMDLK
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Its role in tRNA splicing and maturation is required for cerebellar development. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by dicer1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.
  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    Clp1 family, Clp1 subfamily
  • 數據庫鏈接：

    KEGG: xla:431824

    UniGene: Xl.30457



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