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  4. Recombinant Xenopus laevis Guanine nucleotide-binding protein G (i) subunit alpha-1

Recombinant Xenopus laevis Guanine nucleotide-binding protein G (i) subunit alpha-1

  • 中文名稱：
    有爪蟾蜍gnai1重組蛋白
  • 貨號：
    CSB-YP333220XBE
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    有爪蟾蜍gnai1重組蛋白
  • 貨號：
    CSB-EP333220XBE
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    有爪蟾蜍gnai1重組蛋白
  • 貨號：
    CSB-EP333220XBE-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    有爪蟾蜍gnai1重組蛋白
  • 貨號：
    CSB-BP333220XBE
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    有爪蟾蜍gnai1重組蛋白
  • 貨號：
    CSB-MP333220XBE
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    gnai1Guanine nucleotide-binding protein G(i) subunit alpha-1; Adenylate cyclase-inhibiting G alpha protein
  • 種屬：
    Xenopus laevis (African clawed frog)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    2-354
  • 氨基酸序列
    GCTLSAEDK AAVERSKMID RNLREDGEKA AREVKLLLLG AGESGKSTIV KQMKIIHEAG YSEEECKQYK AVVYSNTIQS IIAIIRAMGR LKIDFGDPSR ADDARQLFVL AGAAEEGFMT AELAGVIKRL WKDGGVQACF NRSREYQLND SAAYYLNDLD RIAQNSYIPT QQDVLRTRVK TTGIVETHFT FKDLHFKMFD VGGQRSERKK WIHCFEGVTA IIFCVALSDY DLVLAEDEEM NRMHESMKLF DSICNNKWFT DTSIILFLNK KDLFEEKIKR SPLTICYPEY PGSNTYEEAA AYIQCQFEDL NKRKDTKEIY THFTCATDTK NVQFVFDAVT DVIIKNNLKD CGLF
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins. Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels. Required for cortical dynein-dynactin complex recruitment during metaphase.
  • 亞細胞定位：
    Nucleus. Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cell cortex. Membrane; Lipid-anchor.
  • 蛋白家族：
    G-alpha family, G(i/o/t/z) subfamily
  • 數據庫鏈接：

    KEGG: xla:380034

    UniGene: Xl.1188



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