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  4. Recombinant Xenopus laevis Double-strand break repair protein MRE11 (mre11), partial

Recombinant Xenopus laevis Double-strand break repair protein MRE11 (mre11), partial

  • 中文名稱：
    有爪蟾蜍mre11重組蛋白
  • 貨號：
    CSB-YP892947XBE
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    有爪蟾蜍mre11重組蛋白
  • 貨號：
    CSB-EP892947XBE
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    有爪蟾蜍mre11重組蛋白
  • 貨號：
    CSB-EP892947XBE-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    有爪蟾蜍mre11重組蛋白
  • 貨號：
    CSB-BP892947XBE
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    有爪蟾蜍mre11重組蛋白
  • 貨號：
    CSB-MP892947XBE
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    mre11; Double-strand break repair protein MRE11; EC 3.1.-.-
  • 種屬：
    Xenopus laevis (African clawed frog)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Involved in DNA double-strand break repair (DSBR). Possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. Also involved in meiotic DSB processing.
  • 基因功能參考文獻：
    1. these findings suggest that the structure of ends is a major determinant for the pathway choice of DSB repair and the Mre11 nuclease dependency of resection. PMID: 27084932
    2. MRN (Mre11, Rad50, and Nbs1) complex, CtIP, and BRCA1 are required for both the removal of Top2-DNA adducts and the subsequent resection of Top2-adducted DSB ends. PMID: 26880199
    3. these data point to Mre11 as an important locus of Rsk-mediated checkpoint inhibition acting upstream of ATM activation. PMID: 24297933
    4. The MRN complex, in particular the nuclease activity of Mre11, plays an important role in the activation of Chk1 in response to stalled replication forks. PMID: 23468519
    5. MRN (MRE11-RAD50-NBS1) complex has role in ATR activation via TOPBP1 recruitment. PMID: 23582259
    6. Results indicate a role for the X. laevis Mre11/Rad50/Nbs1 complex in microhomology-mediated end joining. PMID: 19892829
    7. Mre11 has a role in assembling DNA damage signaling centers PMID: 15138496
    8. These findings suggest that the MRN complex is a crucial mediator in the process whereby ATM promotes the TopBP1-dependent activation of ATR-ATRIP in response to double-stranded DNA breaks. PMID: 19279141

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    MRE11/RAD32 family
  • 數據庫鏈接：

    KEGG: xla:394308

    UniGene: Xl.488



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