在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Woolly monkey sarcoma virus Pol polyprotein (pol)

  • 中文名稱:
    Recombinant Woolly monkey sarcoma virus Pol polyprotein(pol)
  • 貨號(hào):
    CSB-YP356024WBA
  • 說明書:
  • 規(guī)格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    Recombinant Woolly monkey sarcoma virus Pol polyprotein(pol)
  • 貨號(hào):
    CSB-EP356024WBA
  • 說明書:
  • 規(guī)格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    Recombinant Woolly monkey sarcoma virus Pol polyprotein(pol)
  • 貨號(hào):
    CSB-EP356024WBA-B
  • 說明書:
  • 規(guī)格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    Recombinant Woolly monkey sarcoma virus Pol polyprotein(pol)
  • 貨號(hào):
    CSB-BP356024WBA
  • 說明書:
  • 規(guī)格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    Recombinant Woolly monkey sarcoma virus Pol polyprotein(pol)
  • 貨號(hào):
    CSB-MP356024WBA
  • 說明書:
  • 規(guī)格:
  • 來源:
    Mammalian cell
  • 其他:

產(chǎn)品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    pol
  • Uniprot No.:
  • 別名:
    pol; Gag-Pol polyprotein [Cleaved into: Matrix protein p15; MA); RNA-binding phosphoprotein p12; pp12); Capsid protein p30; CA); Nucleocapsid protein p10-Pol; NC-pol); Protease; PR; EC 3.4.23.-); Reverse transcriptase/ribonuclease H; RT; EC 2.7.7.49; EC 2.7.7.7; EC 3.1.26.4; p80); Integrase; IN; EC 2.7.7.-; EC 3.1.-.-; p46)]
  • 種屬:
    Woolly monkey sarcoma virus (WMSV) (Smian sarcoma-associated virus)
  • 蛋白長(zhǎng)度:
    full length protein
  • 表達(dá)區(qū)域:
    1-294
  • 氨基酸序列
    GSLGRGDRPG VYWEVDFTEV KPGRYGNRYL LVFIDTFSGW VEAFPTKTET ALTVCKRNST PLRIPKVLGS DNGPAFVAQV SQGLATQLGI NWKLHCAYRP QSSGQVERMN RTIKETLTKL ALETGGKDWV ALLPLALLRA KNTPSRFGLT PYEILYGGPP PILESGGTLG PDDNFLPVLF THLKALEVVR TQIWDQIKEV YKPGTVAIPH PFQVGDQVLV RRHRPGSLEP RWKGPYLVLL TTPTAVKVDG IAAWVHASHL KPAPPSAPDE SWELEKADHP LKLRIRRRRN ESAK
  • 蛋白標(biāo)簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式:
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng):
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag-Pol, or to Gag-Pol binding host factors. Interaction with HECT ubiquitin ligases probably links the viral protein to the host ESCRT pathway and facilitates release.; Targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the pre-integration complex.; Constituent of the pre-integration complex (PIC) which tethers the latter to mitotic chromosomes. This allows the integration of the viral genome into the host DNA.; Forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex.; Involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved UCUG elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization. Acts as a nucleic acid chaperone which is involved in rearrangement of nucleic acid secondary structures during gRNA retrotranscription.; The aspartyl protease mediates proteolytic cleavages of Gag and Gag-Pol polyproteins during or shortly after the release of the virion from the plasma membrane. Cleavages take place as an ordered, step-wise cascade to yield mature proteins. This process is called maturation. Displays maximal activity during the budding process just prior to particle release from the cell.; RT is a multifunctional enzyme that converts the viral dimeric RNA genome into dsDNA in the cytoplasm, shortly after virus entry into the cell. This enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes in a partially processive 3' to 5' endonucleasic mode. Conversion of viral genomic RNA into dsDNA requires many steps. A tRNA binds to the primer-binding site (PBS) situated at the 5' end of the viral RNA. RT uses the 3' end of the tRNA primer to perform a short round of RNA-dependent minus-strand DNA synthesis. The reading proceeds through the U5 region and ends after the repeated (R) region which is present at both ends of viral RNA. The portion of the RNA-DNA heteroduplex is digested by the RNase H, resulting in a ssDNA product attached to the tRNA primer. This ssDNA/tRNA hybridizes with the identical R region situated at the 3' end of viral RNA. This template exchange, known as minus-strand DNA strong stop transfer, can be either intra- or intermolecular. RT uses the 3' end of this newly synthesized short ssDNA to perform the RNA-dependent minus-strand DNA synthesis of the whole template. RNase H digests the RNA template except for a polypurine tract (PPT) situated at the 5' end of the genome. It is not clear if both polymerase and RNase H activities are simultaneous. RNase H probably can proceed both in a polymerase-dependent (RNA cut into small fragments by the same RT performing DNA synthesis) and a polymerase-independent mode (cleavage of remaining RNA fragments by free RTs). Secondly, RT performs DNA-directed plus-strand DNA synthesis using the PPT that has not been removed by RNase H as primers. PPT and tRNA primers are then removed by RNase H. The 3' and 5' ssDNA PBS regions hybridize to form a circular dsDNA intermediate. Strand displacement synthesis by RT to the PBS and PPT ends produces a blunt ended, linear dsDNA copy of the viral genome that includes long terminal repeats (LTRs) at both ends.; Catalyzes viral DNA integration into the host chromosome, by performing a series of DNA cutting and joining reactions. This enzyme activity takes place after virion entry into a cell and reverse transcription of the RNA genome in dsDNA. The first step in the integration process is 3' processing. This step requires a complex comprising the viral genome, matrix protein and integrase. This complex is called the pre-integration complex (PIC). The integrase protein removes 2 nucleotides from each 3' end of the viral DNA, leaving recessed CA OH's at the 3' ends. In the second step that requires cell division, the PIC enters cell nucleus. In the third step, termed strand transfer, the integrase protein joins the previously processed 3' ends to the 5' ends of strands of target cellular DNA at the site of integration. The last step is viral DNA integration into host chromosome.
  • 亞細(xì)胞定位:
    [Gag-Pol polyprotein]: Virion. Host cell membrane; Lipid-anchor. Host late endosome membrane; Lipid-anchor. Host endosome, host multivesicular body.; [Matrix protein p15]: Virion.; [Capsid protein p30]: Virion.; [Nucleocapsid protein p10-Pol]: Virion.; [Protease]: Virion.; [RNA-binding phosphoprotein p12]: Host cytoplasm.
  • 蛋白家族:
    Retroviral Pol polyprotein family
  • 數(shù)據(jù)庫鏈接:

    KEGG: vg:5176146



主站蜘蛛池模板: 少妇真人直播免费视频| 又黄又爽又色视频免费| 久久精品国产成人午夜福利| 免费刺激性视频大片区| 国产午夜福利伦理300| 人人妻人人爽人人做夜欢视频九色| 欲求不满邻居的爆乳在线播放| 久热这里只有精品99| 高潮迭起av乳颜射后入| 免费人成自慰网站| 国产精品毛片av在线看| 伊人精品成人久久综合| 国产亚洲人成无码网在线观看| 中文字幕av一区二区三区| 美女内射毛片在线看免费人动物| 国产激情无码视频在线播放| 国产婷婷丁香五月缴情成人网| 国产在线精品二区| 亚洲精品日韩一区二区电影| 夜夜躁狠狠躁日日躁202| 亚洲精品美女久久777777| 免费欧洲美女牲交视频| 精品无人区一区二区三区| 欧美性生交活xxxxxdddd| 久久99国产精品久久99| 99精品国产在热久久婷婷| a毛看片免费观看视频| 国产亚洲精品岁国产微拍精品| 久久久久成人片免费观看蜜芽| 国产精品免费一区二区三区四区| 免费视频爱爱太爽了| 国产亚洲日韩妖曝欧美| 久久婷婷成人综合色综合| 成人av片无码免费网站| 亚洲人成人伊人成综合网无码| 最激烈的床震娇喘视频出水| 成人小说亚洲一区二区三区 | 日本xxx在线观看免费播放| 亚洲人成无码网站久久99热国产| 少妇性l交大片7724com | 成人区人妻精品一区二区三区|