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  4. Recombinant Vibrio cholerae serotype O1 GTPase Obg/CgtA (cgtA)

Recombinant Vibrio cholerae serotype O1 GTPase Obg/CgtA (cgtA)

  • 中文名稱：
    Recombinant Vibrio cholerae serotype O1 GTPase Obg/CgtA(cgtA)
  • 貨號：
    CSB-YP881644VEX
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Vibrio cholerae serotype O1 GTPase Obg/CgtA(cgtA)
  • 貨號：
    CSB-EP881644VEX
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Vibrio cholerae serotype O1 GTPase Obg/CgtA(cgtA)
  • 貨號：
    CSB-EP881644VEX-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Vibrio cholerae serotype O1 GTPase Obg/CgtA(cgtA)
  • 貨號：
    CSB-BP881644VEX
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Vibrio cholerae serotype O1 GTPase Obg/CgtA(cgtA)
  • 貨號：
    CSB-MP881644VEX
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    cgtA
  • Uniprot No.：
  • 別名：
    cgtA; obg; VC_0437; GTPase Obg/CgtA; EC 3.6.5.-; CgtA; GTP-binding protein Obg
  • 種屬：
    Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-390
  • 氨基酸序列
    MKFVDEAVIK VQAGDGGNGV VSFWREKFVT NGGPDGGDGG DGGDVYMVAD ENLNTLIDYR FQRFYEAERG KNGGGGNCTG KSGKDKELRV PVGTRAVDIH TNEIIGEVAE HGKKVMIAKG GWHGLGNARF KSSVNRSPRQ KTLGTKGELR DIRLELLLLA DVGMLGMPNA GKSTFIRAVS AAKPKVADYP FTTLVPSLGV VSVLPEKSFV VADIPGLIEG AAEGAGLGIR FLKHLERCRV LLHMIDIMPA DQSDPAHNAL TIIDELEQYS EKLAKKPRWL VFNKVDLMSE EEADEIIQNI IDALAWEGDY FKISAANRQG TKELCMKLAE FMDTLPREAE EKTEAEKVDF TWDYNHKDGL AGREVITEDD DDWDDWDDEE DDGHVIYVRD
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Depletion experiments lead to gene down regulation and a dramatic increase in ppGpp levels, like those seen in the stringent response. There is no change in cell morphology in depletion experiments, but cells are very sensitive to the DNA-damaging agent hydroxyurea and are very elongated. Overexpression reduces growth and leads to elongated cells. Overexpression of proteins with C-terminal deletions of 29 or 62 amino acids showed fewer elongated cells.; An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. It may play a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control. GTPase activity is stimulated by 50S ribosomal subunits.
  • 基因功能參考文獻：
    1. Data show that depletion of CgtA affected the growth of V. cholerae and rendered the cells highly sensitive to the replication inhibitor hydroxyurea. PMID: 18456812
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    TRAFAC class OBG-HflX-like GTPase superfamily, OBG GTPase family
  • 數據庫鏈接：

    KEGG: vch:VC0437

    STRING: 243277.VC0437



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