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  4. Recombinant Vaccinia virus Poly (A) polymerase catalytic subunit

Recombinant Vaccinia virus Poly (A) polymerase catalytic subunit

  • 中文名稱：
    Recombinant Vaccinia virus Poly(A) polymerase catalytic subunit
  • 貨號：
    CSB-YP338514VAI
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Vaccinia virus Poly(A) polymerase catalytic subunit
  • 貨號：
    CSB-EP338514VAI
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Vaccinia virus Poly(A) polymerase catalytic subunit
  • 貨號：
    CSB-EP338514VAI-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Vaccinia virus Poly(A) polymerase catalytic subunit
  • 貨號：
    CSB-BP338514VAI
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Vaccinia virus Poly(A) polymerase catalytic subunit
  • 貨號：
    CSB-MP338514VAI
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    PAPL
  • Uniprot No.：
  • 別名：
    PAPL; VACWR057; E1L; Poly(A) polymerase catalytic subunit; EC 2.7.7.19; Poly(A) polymerase large subunit; PAP-L; VP55
  • 種屬：
    Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-479
  • 氨基酸序列
    MNRNPDQNTL PNITLKIIET YLGRVPSVNE YHMLKLQARN IQKITVFNKD IFVSLVKKNK KRFFSDVNTS ASEIKDRILS YFSKQTQTYN IGKLFTIIEL QSVLVTTYTD ILGVLTIKAP NVISSKISYN VTSMEELARD MLNSMNVAVI DKAKVMGRHN VSSLVKNVNK LMEEYLRRHN KSCICYGSYS LYLINPNIRY GDIDILQTNS RTFLIDLAFL IKFITGNNII LSKIPYLRNY MVIKDENDNH IIDSFNIRQD TMNVVPKIFI DNIYIVDPTF QLLNMIKMFS QIDRLEDLSK DPEKFNARMA TMLEYVRYTH GIVFDGKRNN MPMKCIIDEN NRIVTVTTKD YFSFKKCLVY LDENVLSSDI LDLNADTSCD FESVTNSVYL IHDNIMYTYF SNTILLSDKG KVHEISARGL CAHILLYQML TSGEYKQCLS DLLNSMMNRD KIPIYSHTER DKKPGRHGFI NIEKDIIVF
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Polymerase that creates the 3'-poly(A) tail of mRNA's.
  • 基因功能參考文獻：
    1. Unliganded monomeric VP55 can undergo a ;rocking' motion of the N-terminal domain with respect to the other two domains, which may be ;rigidified' upon processivity factor (VP39) docking. PMID: 23519670
    2. Vaccinia virus poly(A) polymerase (VP55) has been analyzed via hydrogen-deuterium exchange mass spectrometry in the absence and presence of its processivity factor, VP39. PMID: 20469877
    3. there is for VP55 a discontinuous mode of translocation for a non-templated polymerase. PMID: 17488726
  • 蛋白家族：
    Poxviridae poly(A) polymerase catalytic subunit family
  • 數據庫鏈接：

    KEGG: vg:3707514



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