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  4. Recombinant Thioredoxin-1 (trx-1)

Recombinant Thioredoxin-1 (trx-1)

  • 中文名稱：
    秀麗隱桿線蟲trx-1重組蛋白
  • 貨號：
    CSB-YP601002CXY
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲trx-1重組蛋白
  • 貨號：
    CSB-EP601002CXY
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲trx-1重組蛋白
  • 貨號：
    CSB-EP601002CXY-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲trx-1重組蛋白
  • 貨號：
    CSB-BP601002CXY
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲trx-1重組蛋白
  • 貨號：
    CSB-MP601002CXY
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    trx-1
  • Uniprot No.：
  • 別名：
    trx-1; B0228.5; Thioredoxin-1
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-115
  • 氨基酸序列
    MLKRCNFKNQVKYFQSDFEQLIRQHPEKIIILDFYATWCGPCKAIAPLYKELATTHKGII FCKVDVDEAEDLCSKYDVKMMPTFIFTKNGDAIEALEGCVEDELRQKVLEHVSAQ
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Shown to facilitate the reduction of insulin disulfide bonds. Might play a role in the reversible nitrosylation of cysteine residues in target proteins, and thereby contributing to the response to intracellular nitric oxide. Shapes the ASJ sensory neuron biphasic response to nitric oxide (NO) exposure; trans-nitrosylation activity might inhibit calcium flux to the cytoplasm in ASJ neurons when exposed to a NO stimulus, whereas de-nitrosylation activity might promote calcium flux when NO is diminished. By regulating the NO-induced ASJ sensory neuron activity, mediates the avoidance response to NO-producing organisms like P.aeruginosa. Positively regulates life span extension under normal and caloric restriction conditions, dauer formation and the oxidative stress response. Contributes to the down-regulation of expression of the insulin-like neuropeptide daf-28 in the ASJ neurons in a redox-independent fashion, thereby promoting dauer formation. Negatively regulates the nuclear localization of the intestinal skn-1 transcription factor in a p38 MAPK pathway-dependent and redox-independent fashion.
  • 基因功能參考文獻：
    1. Trx-1 regulates intestinal SKN-1 nuclear localization in a cell non-autonomous manner. PMID: 26920757
    2. TRX-1 is a thioredoxin homolog. Worms lacking this gene display reduced life span and sensitivity toward oxidative stress. PMID: 16324156
    3. TRX-1 acts as a fluctuating neuronal signaling modulator within ASJ neurons to monitor the adjustment of neuropeptide expression, including insulin-like proteins, during dauer formation in response to adverse environmental conditions. PMID: 21304598
    4. Dietary restriction activates TRX-1 in ASJ neurons during aging, which in turn triggers TRX-1-dependent mechanisms to extend adult lifespan in the worm. PMID: 21334311
    5. Suppression of the C. elegans thioredoxin orthologs trx-1 and trx-2, involved in the redox chaperone activity of exo-3, overrides the protective effect of cep-1 RNAi treatment on neuronal integrity, neuronal function, mean and maximum life span. PMID: 20346071
    6. Data describe a Caenorhabditis elegans thioredoxin (TRX-1), encoded by the gene B0228.5, that is expressed in ASJ ciliated sensory neurons. PMID: 16387300

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  • 蛋白家族：
    Thioredoxin family
  • 組織特異性：
    Expressed in ASJ and ASI ciliated sensory neurons. Expressed in the intestine (at protein level).
  • 數(shù)據(jù)庫鏈接：

    KEGG: cel:CELE_B0228.5

    STRING: 6239.B0228.5a

    UniGene: Cel.16076



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