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  4. Recombinant Thermus thermophilus SsrA-binding protein (smpB)

Recombinant Thermus thermophilus SsrA-binding protein (smpB)

  • 中文名稱：
    Recombinant Thermus thermophilus SsrA-binding protein(smpB)
  • 貨號：
    CSB-YP823302TNT
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Thermus thermophilus SsrA-binding protein(smpB)
  • 貨號：
    CSB-EP823302TNT
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Thermus thermophilus SsrA-binding protein(smpB)
  • 貨號：
    CSB-EP823302TNT-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Thermus thermophilus SsrA-binding protein(smpB)
  • 貨號：
    CSB-BP823302TNT
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Thermus thermophilus SsrA-binding protein(smpB)
  • 貨號：
    CSB-MP823302TNT
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    smpB
  • Uniprot No.：
  • 別名：
    smpB; TTHA0902; SsrA-binding protein; Small protein B
  • 種屬：
    Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-144
  • 氨基酸序列
    MAPVLENRRA RHDYEILETY EAGIALKGTE VKSLRAGKVD FTGSFARFED GELYLENLYI APYEKGSYAN VDPRRKRKLL LHKHELRRLL GKVEQKGLTL VPLKIYFNER GYAKVLLGLA RGKKAYEKRR EDKKEAVRRA LEEL
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 668
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Required for rescue of stalled ribosomes mediated by trans-translation. Binds to tmRNA (also known as SsrA and 10Sa), required for stable association of tmRNA with ribosomes, probably by bridging tmRNA to 50S subunit. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans-translation.
  • 基因功能參考文獻：
    1. crystal structure of a tmRNA fragment, SmpB and elongation factor Tu bound to the ribosome at 3.2 A; structure shows how SmpB plays role of both anticodon loop of tRNA and portions of mRNA to facilitate decoding in the absence of an mRNA codon PMID: 22422985
    2. SmpB undergoes structural changes indicating an induced fit upon binding to the specific region of tRNA-like domain of tmRNA. PMID: 16428302
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    SmpB family
  • 數據庫鏈接：


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