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  4. Recombinant Thermotoga maritima 5-keto-L-gluconate epimerase (iolO)

Recombinant Thermotoga maritima 5-keto-L-gluconate epimerase (iolO)

  • 中文名稱：
    海棲熱袍菌iolO重組蛋白
  • 貨號：
    CSB-YP893461TNJ
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    海棲熱袍菌iolO重組蛋白
  • 貨號：
    CSB-EP893461TNJ-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    海棲熱袍菌iolO重組蛋白
  • 貨號：
    CSB-BP893461TNJ
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    海棲熱袍菌iolO重組蛋白
  • 貨號：
    CSB-MP893461TNJ
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    iolO
  • Uniprot No.：
  • 別名：
    iolO; TM_0416; 5-keto-L-gluconate epimerase; EC 5.1.3.-; Bifunctional nonphosphorylated sugar isomerase; D-erythrose/D-threose isomerase; L-ribulose 3-epimerase; R3E; Nonphosphorylated sugar 3-epimerase; EC 5.1.3.-; Nonphosphorylated sugar aldose-ketose isomerase; EC 5.3.1.-
  • 種屬：
    Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-270
  • 氨基酸序列
    MKLSLVISTS DAAFDALAFK GDLRKGMELA KRVGYQAVEI AVRDPSIVDW NEVKILSEEL NLPICAIGTG QAYLADGLSL THPNDEIRKK AIERVVKHTE VAGMFGALVI IGLVRGRREG RSYEETEELF IESMKRLLEL TEHAKFVIEP LNRYETDFIN TIDDALRILR KINSNRVGIL ADTFHMNIEE VNIPESLKRA GEKLYHFHVA DSNRWAPGCG HFDFRSVFNT LKEIGYNRYV SVECLPLPGG MEEAAEIAFK TLKELIIKLT
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Catalyzes the reversible epimerization between 5-keto-L-gluconate (5-dehydro-L-gluconate) and D-tagaturonate, and thus probably functions in a myo-inositol degradation pathway together with IolG, IolM and IolN. Is not active on the enantiomer 5-keto-D-gluconate. Was also shown to be a nonphosphorylated sugar isomerase with broad substrate specificity in vitro. Is able to catalyze the reversible C3-epimerization of L-ribulose to L-xylulose, D-ribulose to D-xylulose, D-psicose to D-fructose, and D-tagatose to D-sorbose, with a substrate preference for ketopentoses rather than ketohexoses. Also catalyzes the aldose-ketose isomerization reaction from either D-erythrose or D-threose to D-erythrulose. Exhibits no activity for C4-epimerization of D-tagatose to D-fructose.
  • 基因功能參考文獻：
    1. TM0416 is a putative nonphosphorylated sugar epimerase and catalyzes various C5 and C6 epimerization reactions. PMID: 28258150
  • 蛋白家族：
    Hyi family
  • 數據庫鏈接：

    KEGG: tma:TM0416

    STRING: 243274.TM0416



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