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  4. Recombinant Staphylococcus aureus Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp)

Recombinant Staphylococcus aureus Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp)

  • 中文名稱：
    金黃色釀膿葡萄球菌tsaD重組蛋白
  • 貨號：
    CSB-YP642051FLF
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    金黃色釀膿葡萄球菌tsaD重組蛋白
  • 貨號：
    CSB-EP642051FLF
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    金黃色釀膿葡萄球菌tsaD重組蛋白
  • 貨號：
    CSB-EP642051FLF-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    金黃色釀膿葡萄球菌tsaD重組蛋白
  • 貨號：
    CSB-BP642051FLF
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    金黃色釀膿葡萄球菌tsaD重組蛋白
  • 貨號：
    CSB-MP642051FLF
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    tsaD
  • Uniprot No.：
  • 別名：
    tsaD; gcp; SAOUHSC_02277; tRNA N6-adenosine threonylcarbamoyltransferase; EC 2.3.1.234; N6-L-threonylcarbamoyladenine synthase; t(6)A synthase; t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD; tRNA threonylcarbamoyladenosine biosynthesis protein TsaD
  • 種屬：
    Staphylococcus aureus (strain NCTC 8325)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-341
  • 氨基酸序列
    MTKDILILAV ETSCDETSVS VIKNGRDILS NTVLSQIESH KRFGGVVPEV ASRHHVEGIT ATINEALGDA DVSIEDIDAI AVTEGPGLIG ALLIGVNAAK ALAFAYDKPL IPVHHIAGHI YANHIEEPLT FPLIALIVSG GHTELVYMKD HLSFEVIGET RDDAVGEAYD KVARTIGLNY PGGPQVDRLA AEGEDTYSFP RVWLDKDSYD FSFSGLKSAV INQLHNQRQK NIPIIEANVA TSFQNSVVEV LTFKAIQACK EYGVQRLIVA GGVASNKGLR QSLADQCKVN DIQLTIPSPK LCTDNAAMIG VAGHYLYQQG RFADLALNGH SNIDLEEYSA E
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction. Critical mediator involved in the modification of cell wall peptidoglycan synthesis and/or cell division as well as in the positive regulation of the activities of different murein hydrolases. Essential for cell viability. Negatively affects the expression of lrgA. Positively affects cidA expression, maybe indirectly. May be an important chelator of excess zinc. Down-regulation of gcp/tsaD eliminates penicillin- and vancomycin-caused cell lysis, inhibits several extracellular hydrolase activities, dramatically increasing tolerance to hydrolases and leads to a bacteriostatic effect.
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    KAE1 / TsaD family
  • 數據庫鏈接：


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