在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Staphylococcus aureus Alpha-hemolysin (hly)

In Stock
  • 中文名稱:
    金黃色釀膿葡萄球菌hly重組蛋白
  • 貨號:
    CSB-EP639324FLF
  • 規(guī)格:
    ¥2328
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產(chǎn)品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
    hly
  • Uniprot No.:
  • 別名:
    hly; hla; SAOUHSC_01121Alpha-hemolysin; Alpha-HL; Alpha-toxin
  • 種屬:
    Staphylococcus aureus (strain NCTC 8325)
  • 蛋白長度:
    Full Length of Mature Protein
  • 來源:
    E.coli
  • 分子量:
    49.3kDa
  • 表達區(qū)域:
    27-319aa
  • 氨基酸序列
    ADSDINIKTGTTDIGSNTTVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKSGLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGGLIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYGNQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYERVRDDYQLHWTSTNWKGTNTKDKWIDRSSERYKIDWEKEEMTN
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 6xHis-SUMO-tagged
  • 產(chǎn)品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述:

    To prepare this Recombinant S. aureus hly protein, the recombinant DNA was required, which was generated by fusing the hly gene with N-terminal 6xHis-SUMO tag sequence. Once the recombinant DNA was amplified and purified, a protein expression system, E.coli, was needed for this hly protein production. After purification, a premium hly recombinant proteinwas obtained. According to SDS-PAGE, its purity turns out to be 90%+.

    hly, also known as hla, is a gene encoding a protein named Alpha-hemolysin or Alpha-toxin that is the major cytotoxic agent released by bacterium Staphylococcus aureus. The encoded protein Hla can lyse many types of cells, including human monocytes, lymphocytes, red blood cells, platelets and endothelial cells. It is secreted outside the cell in the form of a soluble monomer, and then aggregates on various cell membranes in the form of a heptamer. Small molecules in the cytoplasm, such as ATP, leak out of the cell through the pores, irreversibly changing the osmotic pressure of the cell. disrupt the integrity of cell membranes.

  • Datasheet & COA:
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能:
    Alpha-toxin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. Inhibits host neutrophil chemotaxis to the lesion region (Probable). Heptamer oligomerization and pore formation is required for lytic activity.
  • 基因功能參考文獻:
    1. We now show that ADAM10 is critical for alpha-hemolysin-mediated activation of the NLRP3 inflammasome in human monocytes as siRNA knockdown or chemical blockade of ADAM10-alpha-hemolysin interaction leads to diminished inflammasome activation and cell death by reducing the available ADAM10 on the cell surface. PMID: 27043625
    2. Staphylococcus aureus hemolysin A disrupts cell-matrix adhesions in human airway epithelial cells. PMID: 24918472
    3. Succeeded in preparing crystals of the heptameric form of alpha-hemolysin without any detergent but with 2-methyl-2,4-pentanediol (MPD), and determined its structure. PMID: 21280135
    4. Staphylococcus aureus alpha-hemolysin activates the NLRP3-inflammasome in human and mouse monocytic cells PMID: 19826485
    5. The wide vestibule leading into the alpha-hemolysin pore helps to orient negatively charged synthetic alpha-helical peptides for translocation. PMID: 16866363

    顯示更多

    收起更多

  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Aerolysin family
  • 數(shù)據(jù)庫鏈接:


主站蜘蛛池模板: 国产未发育呦交视频| 欧洲精品99毛片免费高清观看 | 无码加勒比一区二区三区四区| 国产精品亚韩精品无码a在线| 国产欧美日韩| 亚洲乱码av一区二区| 影视av久久久噜噜噜噜噜三级| 天天天天躁天天爱天天碰2018| 日本新janpanese乱熟| 国产v精品成人免费视频| 2022一本久道久久综合狂躁| 人人插人人插人人爽| 无码人妻aⅴ一区二区三区有奶水| 欧美人禽杂交狂配免费看| 老子影院无码午夜伦不卡| 黑人粗硬进入过程视频| 国产后进白嫩翘臀在线播放| 欧美牲交视频免费观看| 国产又黄又爽又色的免费视频| 少妇高潮惨叫久久久久久| 人妻少妇-嫩草影院| 国产亚洲色欲色一色www| 国产人久久人人人人爽| 日韩成人无码| 久久99亚洲精品久久久久| 国产一在线精品一区在线观看 | 成年女人午夜性视频| 免费网站看sm调教视频| 又黄又爽又无遮挡免费的网站| 久久亚洲中文字幕伊人久久大| 欧美成人精品第一区| 夜夜嗨av一区二区三区| 天天看片视频免费观看| 欧美日产欧美日产国产精品| 国产丰满老熟妇乱xxx1区| 久久9精品区-无套内射无码| 人妻无码中文字幕一区二区三区| 亚洲最大无码中文字幕网站| 熟女性饥渴一区二区三区| 亚洲日韩国产一区二区三区| 2020久久天天躁狠狠躁夜夜|