在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Simian immunodeficiency virus Protein Vpx (vpx)

  • 中文名稱:
    Recombinant Simian immunodeficiency virus Protein Vpx(vpx)
  • 貨號:
    CSB-YP323941SHD
  • 規(guī)格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    Recombinant Simian immunodeficiency virus Protein Vpx(vpx)
  • 貨號:
    CSB-EP323941SHD-B
  • 規(guī)格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    Recombinant Simian immunodeficiency virus Protein Vpx(vpx)
  • 貨號:
    CSB-BP323941SHD
  • 規(guī)格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    Recombinant Simian immunodeficiency virus Protein Vpx(vpx)
  • 貨號:
    CSB-MP323941SHD
  • 規(guī)格:
  • 來源:
    Mammalian cell
  • 其他:

產(chǎn)品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    vpx
  • Uniprot No.:
  • 別名:
    vpxProtein Vpx; Viral protein X; X ORF protein
  • 種屬:
    Simian immunodeficiency virus (isolate PBj14/BCL-3) (SIV-sm) (Simian immunodeficiency virus sooty mangabey monkey)
  • 蛋白長度:
    full length protein
  • 表達區(qū)域:
    1-112
  • 氨基酸序列
    MSDPRERIPP GNSGEETIGE AFDWLDRTVE EINRAAVNHL PRELIFQVWR RSWEYWHDEM GMSVSYTKYR YLCLIQKAMF MHCKKGCRCL GGEHGAGGWR PGPPPPPPPG LA
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產(chǎn)品評價

問答及客戶評論

 常見問題解答
Q:

I have purchased your vpx recombinant protein a long time ago (CSB-EP323941SHD; Lot#: E051453) and I would like to see the COA for the batch we received. Can you please provide this COA. Also, was the protein sent to us liquid or lyophilized? Please provide the formulation of the buffer that was used as well as Endotoxin level (if tested).

A:
Very nice to receive your inquiry. I have checked your order record and the details are as below.
Name: Recombinant Simian immunodeficiency virus Protein Vpx (vpx)
CUSABIO Code: CSB-EP323941SHD
Order Time: September 5th, 2014
Size: 5mg
Seq Pos: 1-112
Sequence:

MSDPRERIPPGNSGEETIGEAFDWLDRTVEEINRAAVNHLPRELIFQVWRRSWEYWHDEMGMSVSYTKYR YLCLIQKAMFMHCKKGCRCLGGEHGAGGWRPGPPPPPPPGLA


Tag Information: His tagged
Unit Size: 5 mg (E Coli Derived)
This protein was delivered in year 2014. We didn't provide COA at that time and could only provide datasheet (attached).
The formulation of the buffer is 20mM Tris-HCl, 0.5M NaCl, PH 8.0,50% glycerol.
You didn't require endotoxin removal when placing the order, so didn't test the endotoxin level.
Q:

We recently received your protein CSB-EP323941SHD, lot 03767, which was concerned because this lot was more concentrated than the previous lots they received. We want to verify the concentration, and were wondering if you had a recommended commercial assay that we would be able to purchase to confirm the concentration found by your team. Would you be able to advise on the Bradford kit you guys used to determine the concentration of this lot?

A:
Thanks for your inquiry. As shown in our Datasheet&COA, the method we used to determine the protein concentration is Bradford Method, and the standard we used is BSA.
We determined this group of solutions by formulating a set of bovine serum albumin (BSA) solutions at concentrations of 0.10 mg/ml, 0.08 mg/ml, 0.06 mg/ml, 0.04 mg/ml, 0.02 mg/ml, and 0 mg/ml, respectively.
The absorbance gives a standard curve of protein concentration versus absorbance. The absorbance of the sample of unknown protein concentration was determined, and the concentration of the protein was
obtained according to a standard curve. At the same time, we also combined SDS-PAGE observations to determine the target protein concentration.
For your problem "Lot03767 was more concentrated than the previous lots we received". Would you please confirm which lots you had received before?
Q:

Lot# 03767 concentration question. Hello, My Certificate of analysis for this lot says 0.3 mg/ml. This is my third lot that I have received in the past year, and this lot seems to be more potent than the other two lots when run in the same assay. I see that you test concentration by using the Bradford Method. I have two topics that I would like to know more about: 1) Do you have more specific data for this lot--was it closer to 0.34 mg/mL--or does this assay only give results to one significant figure? 2) Do you use a commercial test kit to measure the concentration? I see that Thermal Fisher Life Technologies has several types of test kits made by Pierce available. Do you know if their cat #23236 Coomassie Plus (Bradford) Assay Kit will give similar concentration results as what you perform? Do you have a suggestion of a commercial test kit that I could use to check the protein concentration myself? Thank you for your help with this inquiry.
Current lot→0.3mg/ml
Lot# 03614→0.8mg/ml
Lot# 03433→0.4mg/ml

A:
Thanks for your quick response.
1. Yes. The result gives an approximation that is not a very certain value.
So whether it is close to 0.34 mg/ml, there is such a possibility, but we can't confirm it yet, and there may be some deviations in different concentration detection methods.
2. We did not use a commercial test kit to measure the concentration.
If you want to verify, you can buy a commercial test kit to detect relevant protein concentration, and we will be grateful if you finally share the test results with us.

靶點詳情

  • 功能:
    Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells. Targets specific host proteins for degradation by the 26S proteasome. Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host VPRPB/DCAF-1. This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1. In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription.
  • 亞細胞定位:
    Virion. Host nucleus.
  • 蛋白家族:
    Lentivirus VPX protein family


主站蜘蛛池模板: 欧美 日产 国产在线观看| 国产欧洲精品自在自线官方| 欧美国产日韩亚洲中文| 中文无码精品a∨在线| 羞羞影院午夜男女爽爽| 天堂8在线天堂资源bt| 男女猛烈xx00免费视频试看| 久久人妻无码一区二区| 一边捏奶头一边啪高潮视频| 成人片黄网站a毛片免费观看 | 中文精品久久久久国产网址| 亚洲乱码日产精品bd在线观看| 九九视频国产免| 亚洲成在线aⅴ免费视频| 日本日本乱码伦专区| 久久精品国产亚洲7777| 久久久久欧美精品| 精品亚洲一区二区三区在线播放 | 伊人色综合九久久天天蜜桃| 精品精品国产高清a毛片| 在厨房被c到高潮a毛片奶水| 亚洲成a人片在线观看无码下载| 久久精品囯产精品亚洲| 久久精品国产2020| 99久久人妻无码精品系列蜜桃| 亚洲精品92内射| 亚洲色在线v中文字幕| 日韩av无码中文无码电影| 欧美日本精品一区二区三区| 青青草国产成人99久久| 亚洲另类无码专区国内精品| 亚洲熟妇av一区二区三区浪潮| 337p日本欧洲亚洲大胆在线| 97久久精品亚洲中文字幕无码| 亚洲国产精品久久久久婷蜜芽| 少妇一边呻吟一边说使劲| 男人边吃奶边做好爽免费视频| 免费人成自慰网站| 色五月丁香五月综合五月4438| 国产高清乱理伦片| 丰满少妇在线观看网站|