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  4. Recombinant Sheep Lens fiber major intrinsic protein (MIP), partial

Recombinant Sheep Lens fiber major intrinsic protein (MIP), partial

  • 中文名稱：
    綿羊MIP重組蛋白
  • 貨號：
    CSB-YP764270SH1
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    綿羊MIP重組蛋白
  • 貨號：
    CSB-EP764270SH1
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    綿羊MIP重組蛋白
  • 貨號：
    CSB-EP764270SH1-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    綿羊MIP重組蛋白
  • 貨號：
    CSB-BP764270SH1
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    綿羊MIP重組蛋白
  • 貨號：
    CSB-MP764270SH1
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MIP
  • Uniprot No.：
  • 別名：
    MIP; AQP0; Lens fiber major intrinsic protein; Aquaporin-0
  • 種屬：
    Ovis aries (Sheep)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Water channel. Channel activity is down-regulated by CALM when cytoplasmic Ca(2+) levels are increased. May be responsible for regulating the osmolarity of the lens. Interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core. Plays a role in cell-to-cell adhesion and facilitates gap junction coupling.
  • 基因功能參考文獻：
    1. Role of Pore-Lining Residues in Defining the Rate of Water Conduction by Aquaporin-0 PMID: 28297654
    2. electron crystallographic structure determination of the AQP0-mediated membrane junction, is reported. PMID: 25635393
    3. Through the online combination of size exclusion chromatography, small-angle X-ray scattering , and refractometry, the study determined a precise geometrical model of the n-dodecyl beta-d-maltopyranoside corona surrounding aquaporin-0. PMID: 22621369
    4. 1.9 A resolution structure of junctional AQP0, determined by electron crystallography of double-layered two-dimensional crystals. PMID: 16319884
    5. The first structural models of the supramolecular organization of AQP0 tetramers & connexon hexamers in junctional microdomains were generated by docking atomic models of AQP0 and connexons into the atomic force microscopy topographs. PMID: 17869130

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  • 亞細胞定位：
    Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
  • 蛋白家族：
    MIP/aquaporin (TC 1.A.8) family
  • 組織特異性：
    Detected in eye lens (at protein level).
  • 數據庫鏈接：

    KEGG: oas:100294602

    UniGene: Oar.19891



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