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Recombinant Sheep Fibroblast growth factor 2 (FGF2) (Active)

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  • 品名簡稱:
    Recombinant Sheep FGF2 protein (Active)
  • 貨號:
    CSB-YP008625SH
  • 說明書:
  • 規格:
    ¥1656
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Activity
      The ED50 as determined by the dose-dependent stimulation of the proliferation of NIH-3T3 cells is 3.791-5.569 ng/mL.
  • 其他:

產品詳情

  • 純度:
    Greater than 95% as determined by SDS-PAGE.
  • 內毒素:
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性:
    The ED50 as determined by the dose-dependent stimulation of the proliferation of NIH-3T3 cells is 3.791-5.569 ng/mL.
  • 基因名:
  • Uniprot No.:
  • 別名:
    Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor (bFGF); Heparin-binding growth factor 2 (HBGF-2); FGF2
  • 種屬:
    Ovis aries (Sheep)
  • 蛋白長度:
    Full Length of Mature Protein
  • 來源:
    Yeast
  • 分子量:
    17.9 kDa
  • 表達區域:
    10-155aa
  • 氨基酸序列
    PALPEDGGSSAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYSSWYVALKRTGQYKLGPKTGPGQKAILFLPMSAKS
  • 蛋白標簽:
    C-terminal 6xHis-tagged
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation.
  • 基因功能參考文獻:
    1. FGF2 mRNA expression was higher in immature and mature oocytes and gradually decreases from 2-cell stage of embryo to the blastocyst stage; a significant differences in FGF2 mRNA expression were observed between immature oocytes and all pre-implantation stages of embryo. PMID: 29638025
    2. this study demonstrated the presence of fibroblast growth factor 2 in ovine ovaries and that it inhibits apoptosis and promotes ovine follicle growth PMID: 24750547
    3. These results suggest that bFGF, VEGF-C and PDGF-C have roles in the pathogenesis of ovine pulmonary adenomatosis. PMID: 22721818
    4. proximal fibroblast growth factor (FGF2/FGFR1) signaling is compartmentalized in the placental endothelial caveolae via the FGFR substrate 2alpha that mediates formation of a FRS2alpha-GRB2-GAB1 complex PMID: 22674390
    5. Exogenous human FGF-2 increased endogenous FGF-2 promoter activity and protein levels in ovine pulmonary arterial smooth muscle cells. PMID: 17942638
    6. Reduced fetal and placental weights in twins and/or triplet pregnancies are associated with an overall decrease in total placental vascularity, VEGF and FGF2 and/or FGFR protein expression. PMID: 18392787

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  • 亞細胞定位:
    Secreted. Nucleus.
  • 蛋白家族:
    Heparin-binding growth factors family
  • 數據庫鏈接:

    KEGG: oas:443306

    UniGene: Oar.394



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