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  4. Recombinant Schizosaccharomyces pombe Vacuolar-sorting protein dot2 (dot2)

Recombinant Schizosaccharomyces pombe Vacuolar-sorting protein dot2 (dot2)

  • 中文名稱：
    龐貝裂殖酵母dot2重組蛋白
  • 貨號：
    CSB-YP529719SXV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母dot2重組蛋白
  • 貨號：
    CSB-EP529719SXV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母dot2重組蛋白
  • 貨號：
    CSB-EP529719SXV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    龐貝裂殖酵母dot2重組蛋白
  • 貨號：
    CSB-BP529719SXV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母dot2重組蛋白
  • 貨號：
    CSB-MP529719SXV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    dot2
  • Uniprot No.：
  • 別名：
    dot2; SPBC651.05c; Vacuolar-sorting protein dot2; Defective organization of telomere protein 2; ELL-associated protein of 30 kDa homolog dot2
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-252
  • 氨基酸序列
    MRKRIGIGAL NDDEYLKQYE EVGNELIEQQ SDEIASQLST FQEALKTFAR EHATEIKQNS QFRNTFVKLA LKIGLDPFVS GSDESAWAAV GMNEFYYQVA VRVIEVCYAT QMENGGLLSV SQVCRFLNEE NEAFGHEWLR ETDVVRAVDS LAPLGPGFVL EKIAGKQYIR SLPLELNTDQ NVVLEAVEIL GYVTISILRD NYAWERSRCI QVLNDLVSKS LLWIDSQGVE MAYWGASNLI DDQAQRFLYE NF
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Negatively regulates meiotic spindle pole body maturation via indirect regulation of the pcp1 gene. Required for efficient entry into pre-meiotic S phase.
  • 基因功能參考文獻：
    1. Dot2, the fission yeast homolog of the human transcription factor EAP30, negatively regulates meiotic spindle pole body maturation. [dot2] PMID: 15992541
  • 亞細胞定位：
    Cytoplasm. Nucleus. Endosome membrane; Peripheral membrane protein.
  • 蛋白家族：
    SNF8 family
  • 數據庫鏈接：


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