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  4. Recombinant Schizosaccharomyces pombe Rho1 guanine nucleotide exchange factor 1 (rgf1), partial

Recombinant Schizosaccharomyces pombe Rho1 guanine nucleotide exchange factor 1 (rgf1), partial

  • 中文名稱：
    龐貝裂殖酵母rgf1重組蛋白
  • 貨號：
    CSB-YP896575SXV
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母rgf1重組蛋白
  • 貨號：
    CSB-EP896575SXV
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母rgf1重組蛋白
  • 貨號：
    CSB-EP896575SXV-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    龐貝裂殖酵母rgf1重組蛋白
  • 貨號：
    CSB-BP896575SXV
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母rgf1重組蛋白
  • 貨號：
    CSB-MP896575SXV
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    rgf1
  • Uniprot No.：
  • 別名：
    rgf1; SPCC645.07; Rho1 guanine nucleotide exchange factor 1
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Stimulates the exchange of Rho1 and Rho5 GDP-bound form into GTP-bound form. Controls septum formation, cell wall synthesis and localization of F-actin patches. Coordinates actin deposition with cell wall biosynthesis during bipolar growth.
  • 基因功能參考文獻：
    1. Rgf1 is involved in cell wall and septum formation. PMID: 16324155
    2. Rgf1p probably activates the Rho1 functions necessary for coordinating actin deposition with cell wall biosynthesis during bipolar growth, allowing the cells to remodel their wall without risk of rupture. PMID: 16421249
    3. Results show that Rgf1p, a Rho1p-specific GEF, acts upstream from the Pck2-Pmk1p MAPK cell integrity signaling pathway. PMID: 19037094
    4. Rgf1p and Rgf2p are alternative guanine nucleotide exchange factors with an essential overlapping function in Rho1p activation during vegetative growth. PMID: 19189958
  • 亞細胞定位：
    Cytoplasm. Note=Septum. Localizes to cell tips during interphase and the septum in mitotic cells.
  • 數(shù)據(jù)庫鏈接：


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