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  4. Recombinant Schizosaccharomyces pombe Poly (A) ribonuclease pop2

Recombinant Schizosaccharomyces pombe Poly (A) ribonuclease pop2

  • 中文名稱：
    Recombinant Schizosaccharomyces pombe Poly(A) ribonuclease pop2
  • 貨號：
    CSB-YP527964SXV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Schizosaccharomyces pombe Poly(A) ribonuclease pop2
  • 貨號：
    CSB-EP527964SXV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Schizosaccharomyces pombe Poly(A) ribonuclease pop2
  • 貨號：
    CSB-EP527964SXV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Schizosaccharomyces pombe Poly(A) ribonuclease pop2
  • 貨號：
    CSB-BP527964SXV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Schizosaccharomyces pombe Poly(A) ribonuclease pop2
  • 貨號：
    CSB-MP527964SXV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    caf1
  • Uniprot No.：
  • 別名：
    caf1; pop2; SPCC18.06cPoly(A) ribonuclease pop2; EC 3.1.13.4; CCR4-associated factor 1
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-335
  • 氨基酸序列
    MTAMNSNFSY PALGVDGISS QISPIRDVWS TNLQQEMNLI MSLIERYPVV SMDTEFPGVV ARPLGVFKSS DDYHYQTLRA NVDSLKIIQI GLALSDEEGN APVEACTWQF NFTFNLQDDM YAPESIELLT KSGIDFKKHQ EVGIEPADFA ELLIGSGLVL QEEVTWITFH SGYDFAYLLK AMTQIPLPAE YEEFYKILCI YFPKNYDIKY IMKSVLNNSK GLQDIADDLQ IHRIGPQHQA GSDALLTARI FFEIRSRYFD GSIDSRMLNQ LYGLGSTGSV LWHNNSSTPQ IQFRDLPGAH PSPTPSNAGI PTTLTNTSSA PNFANSTFRF PPRVV
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Acts as the catalytic component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. In vivo and in vitro, caf1 has 3'-exoribonuclease activity with a preference for poly(A) RNAs.
  • 基因功能參考文獻：
    1. During replication-coupled DNA repair, CAF-1 promotes Rad51-dependent template switches at replication forks, independently of the postreplication repair pathway. PMID: 25313826
    2. The 1.4-A crystal structure of the Pop2 deadenylase subunit reveals a fully conserved DEDDh active site, and allowed for a detailed analysis of its configuration, including divalent metal ion binding. PMID: 17452359
    3. crystallographic analysis of the apo-form of fission yeast Asf1/Cia1 (SpAsf1N; residues 1-161) as well as its complexes with the B-domain of the fission yeast HIRA orthologue Hip1 (Hip1B) and the C-terminal region of the Cac2 subunit of CAF-1 (Cac2C) PMID: 18334479
  • 亞細胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    CAF1 family
  • 數據庫鏈接：


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