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  4. Recombinant Schizosaccharomyces pombe Mitotic spindle checkpoint component mad2 (mad2)

Recombinant Schizosaccharomyces pombe Mitotic spindle checkpoint component mad2 (mad2)

  • 中文名稱：
    龐貝裂殖酵母mad2重組蛋白
  • 貨號：
    CSB-YP519398SXV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母mad2重組蛋白
  • 貨號：
    CSB-EP519398SXV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母mad2重組蛋白
  • 貨號：
    CSB-EP519398SXV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    龐貝裂殖酵母mad2重組蛋白
  • 貨號：
    CSB-BP519398SXV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母mad2重組蛋白
  • 貨號：
    CSB-MP519398SXV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    mad2
  • Uniprot No.：
  • 別名：
    mad2; SPBC20F10.06Mitotic spindle checkpoint component mad2
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-203
  • 氨基酸序列
    MSSVPIRTNF SLKGSSKLVS EFFEYAVNSI LFQRGIYPAE DFKVVRKYGL NMLVSVDEEV KTYIRKIVSQ LHKWMFAKKI QKLILVITSK CSGEDLERWQ FNVEMVDTAD QFQNIGNKED ELRVQKEIQA LIRQITATVT FLPQLEEQCT FNVLVYADKD SEVPTDWVDS DPRILRDAEQ VQLRSFSTSM HKIDCQVAYR VNP
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. It interacts with the anaphase promoting complex/cyclosome (APC/C) thereby inhibiting APC/C-dependent proteolysis, a step required for exit from mitosis.
  • 基因功能參考文獻：
    1. Results provide evidence that the truncation of two beta strands (beta7 and beta8) of Mad2 destabilize the safety belt and affect the Cdc20-Mad2 interaction leading to defects in the spindle checkpoint activation. PMID: 27664031
    2. Mad2p and Slp1p act downstream of Rad3p in the DNA replication checkpoint and Mad2p is required for the DNA replication checkpoint when Cds1p is compromised PMID: 15347659
    3. Mis6-complex, in collaboration with the Nuf2-complex, monitors the spindle-kinetochore attachment state and acts as a platform for Mad2 to accumulate at unattached kinetochores PMID: 15930132
    4. findings indicate that altered Mad2p anaphase spindle localizations can reflect changes in spindle function during mitotic exit that could contribute to fidelity in anaphase events PMID: 16855399
    5. When overexpressed ectopically, mutant Mad2 proteins sequestered wild-type Mad2, preventing its function in mitotic checkpoint arrest, but not in minichromosome stability. PMID: 17277378
    6. These results suggest that CK2 has a role in the spindle checkpoint by regulating Mad2p. PMID: 19666000

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    MAD2 family
  • 數據庫鏈接：


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