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  4. Recombinant Schizosaccharomyces pombe Histone H2A.Z (pht1)

Recombinant Schizosaccharomyces pombe Histone H2A.Z (pht1)

  • 中文名稱：
    龐貝裂殖酵母pht1重組蛋白
  • 貨號：
    CSB-YP010100SXV
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母pht1重組蛋白
  • 貨號：
    CSB-EP010100SXV
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母pht1重組蛋白
  • 貨號：
    CSB-EP010100SXV-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    龐貝裂殖酵母pht1重組蛋白
  • 貨號：
    CSB-BP010100SXV
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母pht1重組蛋白
  • 貨號：
    CSB-MP010100SXV
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    pht1
  • Uniprot No.：
  • 別名：
    pht1; pi001; SPBC11B10.10c; Histone H2A.Z
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-139
  • 氨基酸序列
    MSGGGKGKHV GGKGGSKIGE RGQMSHSARA GLQFPVGRVR RFLKAKTQNN MRVGAKSAVY SAAVLEYLTA EVLELAGNAA KDLKVKRITP RHLQLAIRGD EELDTLIRAT IAGGGVLPHI NKQLLIRTKE KYPEEEEII
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Variant histone H2A which can replace H2A in some nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Important for chromosomal structure and function, possibly including a role in controlling the fidelity of chromosomal segregation during mitosis. Plays an important role in maintaining a silenced chromatin state at centromeres and in the architecture of anaphase chromosomes.
  • 基因功能參考文獻：
    1. Mod22-1, a modifier of microtubule nucleation phenotypes, encodes a truncation allele of Swr1, a chromatin-remodelling factor involved in nucleosomal deposition of H2A.Z histone variant Pht1. PMID: 19160458
    2. Deletion or an unacetylatable mutation of Pht1 leads to genome instability, primarily caused by chromosome entanglement and breakage at anaphase. PMID: 19915592
  • 亞細胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Histone H2A family
  • 數據庫鏈接：


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