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  4. Recombinant Schizosaccharomyces pombe F-box DNA helicase protein 1 (fbh1), partial

Recombinant Schizosaccharomyces pombe F-box DNA helicase protein 1 (fbh1), partial

  • 中文名稱：
    龐貝裂殖酵母fbh1重組蛋白
  • 貨號：
    CSB-YP892017SXV
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母fbh1重組蛋白
  • 貨號：
    CSB-EP892017SXV
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母fbh1重組蛋白
  • 貨號：
    CSB-EP892017SXV-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    龐貝裂殖酵母fbh1重組蛋白
  • 貨號：
    CSB-BP892017SXV
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    龐貝裂殖酵母fbh1重組蛋白
  • 貨號：
    CSB-MP892017SXV
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    fbh1
  • Uniprot No.：
  • 別名：
    fbh1; fdh; fdh1; SPBC336.01F-box DNA helicase protein 1; EC 3.6.4.12
  • 種屬：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Involved in ATP-dependent DNA-unwinding in a 3' to 5' direction, and ATP-ase activities stimulated by the single-stranded DNA-binding protein ssb1. Essential for viability and normal growth of stationary phase cells and in the absence of either srs2 or rqh1 DNA helicase. Involved in DNA recombination repair of strand breaks and stalled or collapsed replication forks, on the rhp51-dependent pathway: promotes rhp51 filament dissolution from stalled forks, thereby inhibiting homologous recombination and preventing excessive recombination. Ubiquitination and DNA helicase activities are essential for controlling rhp51-dependent recombination in the absence of rad22. Plays a role in the processing of toxic recombination intermediates. Promotes proper chromosome segregation.
  • 基因功能參考文獻：
    1. we tentatively suggest that Fbh1 might also have a pro-recombination role in vivo, because the Fbh1-Skp1 complex stimulated Rad51-mediated strand exchange in vitro PMID: 25165823
    2. SCF/Skp1 together with Fbh1 is likely to function in the resolution of meiotic recombination intermediates, thereby ensuring proper chromosome segregation. PMID: 22292001
    3. Fbh1 also serves a vital meiotic role in fission yeast to promote normal chromosome segregation. PMID: 21149262
    4. a version of Atf1 lacking all intact MAPK sites still interacts with Fbh1 upon stress, indicating that the association between the F box protein and substrate is disrupted by stress-induced phosphorylation PMID: 19836238
    5. The F-box DNA helicase Fbh1 functions in recombination repair in the Rhp51 pathway downstream of Rhp51 and Rhp57, and plays a role in processing recombination intermediates. [fbh1] PMID: 16135799
    6. Mediator proteins are required to enable Rhp51 to function in the presence of Fbh1. Analysis of fbh1 mutant alleles suggests that both the F-box and helicase activities of Fbh1 contribute to controlling Rhp51 action. [fbh1] PMID: 16135800
    7. Data show that the F-box DNA helicase (Fbh1) functions in direct opposition to the Rad52 orthologue Rad22 to curb Rad51 loading onto DNA in fission yeast. PMID: 19546232

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  • 亞細胞定位：
    Cytoplasm. Nucleus. Note=Becomes localized at discrete nuclear foci together with rhp51, in response to DNA strand breakage.
  • 蛋白家族：
    Helicase family, UvrD subfamily
  • 數據庫鏈接：


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