在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Salmonella typhimurium Virulence sensor histidine kinase PhoQ (phoQ), partial

In Stock
  • 貨號:
    CSB-EP318475SXB
  • 規(guī)格:
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產(chǎn)品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
    phoQ
  • Uniprot No.:
  • 種屬:
    Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
  • 蛋白長度:
    partial
  • 來源:
    E.coli
  • 分子量:
    38.5 kDa
  • 表達(dá)區(qū)域:
    215-487aa
  • 氨基酸序列
    WWSLRPIEALAREVRELEDHHREMLNPETTRELTSLVRNLNQLLKSERERYNKYRTTLTDLTHSLKTPLAVLQSTLRSLRNEKMSVSKAEPVMLEQISRISQQIGYYLHRASMRGSGVLLSRELHPVAPLLDNLISALNKVYQRKGVNISMDISPEISFVGEQNDFVEVMGNVLDNACKYCLEFVEISARQTDDHLHIFVEDDGPGIPHSKRSLVFDRGQRADTLRPGQGVGLAVAREITEQYAGQIIASDSLLGGARMEVVFGRQHPTQKEE
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽:
    N-terminal 10xhis-tagged and C-terminal myc-tagged
  • 產(chǎn)品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 復(fù)溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能:
    Member of the two-component regulatory system PhoP/PhoQ which regulates the expression of genes involved in virulence, adaptation to acidic and low Mg(2+) environments and resistance to host defense antimicrobial peptides. Essential for intramacrophage survival of S.typhimurium. In low periplasmic Mg(2+), PhoQ functions as a membrane-associated protein kinase that undergoes autophosphorylation and subsequently transfers the phosphate to PhoP, resulting in the expression of PhoP-activated genes (PAG) and repression of PhoP-repressed genes (PRG). In high periplasmic Mg(2+), acts as a protein phosphatase that dephosphorylates phospho-PhoP, resulting in the repression of PAG and may lead to expression of some PRG. Essential for transcription of spiC inside macrophages by controlling the expression of the two-component regulatory system SsrB/SpiR (SsrA) and Pir at transcriptional and post-transcriptional levels respectively. Promotes expression of the two-component regulatory system PmrA/PmrB via activation of pmrD gene. Is required to attenuate bacterial growth within fibroblast cells and to enhance bacterial resistance to bile in intestinal cells. Negatively regulates prgH, which is required for invasion of epithelial cells. Involved in acid tolerance.
  • 基因功能參考文獻:
    1. These data suggest that when Salmonella experiences mildly acidic pH, the virulence regulator PhoP activation requires the sensor protein PhoQ to detect pH and the Salmonella-specific protein UgtL to amplify the PhoQ response. PMID: 28851823
    2. Salmonella enterica Typhimurium, bearing the acidic pH and divalent cation unresponsive phoQ(W104C-A128C) allele is virulent in mice, indicating that acidic pH and divalent cation sensing by PhoQ are dispensable for virulence. PMID: 26002083
    3. Changes in the periplasmic domain of PhoQ lead to conformational movements in the HAMP domain helices which disrupt interaction between the HAMP and the catalytic domains, thus promoting increased kinase activity. PMID: 26015499
    4. study describes a mechanism whereby the transcriptional activator PhoP elicits expression of dissimilar gene sets when its cognate sensor PhoQ is activated by different signals in the periplasm PMID: 24256574
    5. These data demonstrated that Salmonella Typhimurium PhoQ can sense cationic antimicrobial peptides and CRAMP serves as a putative direct PhoPQ activation signal in the mouse intestine. PMID: 22919691
    6. PhoP/Q regulon enables S. Typhimurium to adapt to intramacrophage stresses other than phagolysosomal fusion. PMID: 21511762
    7. RstB-induction increases the levels of phoP mRNA as well as PhoP protein, while lack of the phoPQ genes abolishes RstB-promoted transcription of the PhoP-regulated genes PMID: 20811812
    8. Enzymatic activity of Salmonella typhimurium PhoQ is directly activated by [host] antimicrobial peptides. Our findings reveal a mechanism by which bacteria sense small innate immune molecules to initiate a transcriptional program that promotes virulence PMID: 16096064
    9. induction of PhoP/PhoQ system results in initial surge of PhoP phosphorylation, occupancy of target promoters by PhoP & transcription of PhoP-activated genes; surge by PhoP's positive feedback loop is necessary to jump-start virulence program PMID: 17158330
    10. analysis of signal-dependent regulation in the PhoP/PhoQ system PMID: 18434315
    11. Mutational analysis and NMR spectroscopy of the periplasmic domains of Salmonella typhimurium PhoQ and Pseudomonas aeruginosa PhoQ indicate distinct mechanisms of binding divalent cation. PMID: 18532985
    12. SlyA has a role as a mediator in signal transduction from the PhoP/PhoQ system to other bacterial two-component systems in Salmonella PMID: 18678876
    13. Data report that the PhoP/PhoQ system regulates different Salmonella genes depending on whether the inducing signal is acidic pH or low Mg(2+). PMID: 19801407

    顯示更多

    收起更多

  • 亞細(xì)胞定位:
    Cell inner membrane; Multi-pass membrane protein.
  • 數(shù)據(jù)庫鏈接:

    KEGG: stm:STM1230

    STRING: 99287.STM1230



主站蜘蛛池模板: 日本牲交大片无遮挡| 人人妻人人澡人人爽超污| 精品人妻少妇嫩草av无码专区| 欧美又粗又大又黄的片| 美女露出奶头扒开尿口视频直播| 国产成人综合在线视频| 18精品久久久无码午夜福利| 亚洲国产aⅴ成人精品无吗 | 日本熟妇色xxxxxhd| 欧美人与动牲交app视频| 色诱久久久久综合网ywww| 国产精品无码翘臀在线观看| 小sao货水好多真紧h无码视频| 色九月亚洲综合网| 精品国产av一区二区果冻传媒| 亚洲精品国偷拍自产在线麻豆| 精品人妻人人做人人爽 | 色av综合av综合无码网站| 国产69精品久久久久久妇女迅雷| 久久国产免费观看精品a片| 国产精品久久久久9999高清| 日日碰狠狠躁久久躁蜜桃| 亚洲精品国产成人一区二区| a欧美爰片久久毛片a片| 特黄特色大片免费播放器图片| 色婷婷综合久久久久中文字幕| 精品国产你懂的在线观看 | 999久久久无码国产精品| 国产做a爰片久久毛片a片| 玩弄japan白嫩少妇hd| 蜜臀久久99精品久久久久久做爰| 国产人成无码视频在线1000| 老熟女五十路乱子交尾中出一区| 免费无码a片一区二三区| 免费永久在线观看黄网站| 国产免费无码一区二区三区| 日本三级理论久久人妻电影| 人人澡人人妻人人爽人人蜜桃麻豆 | 久久国产高潮流白浆免费观看| 国产成人av无码精品| 亚洲午夜久久久久妓女影院|