在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Salmonella typhimurium Virulence sensor histidine kinase PhoQ (phoQ), partial

In Stock
  • 中文名稱:
    鼠傷寒沙門菌phoQ重組蛋白
  • 貨號:
    CSB-EP318475SXB
  • 規格:
    ¥2328
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
    phoQ
  • Uniprot No.:
  • 種屬:
    Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
  • 蛋白長度:
    partial
  • 來源:
    E.coli
  • 分子量:
    38.5 kDa
  • 表達區域:
    215-487aa
  • 氨基酸序列
    WWSLRPIEALAREVRELEDHHREMLNPETTRELTSLVRNLNQLLKSERERYNKYRTTLTDLTHSLKTPLAVLQSTLRSLRNEKMSVSKAEPVMLEQISRISQQIGYYLHRASMRGSGVLLSRELHPVAPLLDNLISALNKVYQRKGVNISMDISPEISFVGEQNDFVEVMGNVLDNACKYCLEFVEISARQTDDHLHIFVEDDGPGIPHSKRSLVFDRGQRADTLRPGQGVGLAVAREITEQYAGQIIASDSLLGGARMEVVFGRQHPTQKEE
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 10xhis-tagged and C-terminal myc-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Member of the two-component regulatory system PhoP/PhoQ which regulates the expression of genes involved in virulence, adaptation to acidic and low Mg(2+) environments and resistance to host defense antimicrobial peptides. Essential for intramacrophage survival of S.typhimurium. In low periplasmic Mg(2+), PhoQ functions as a membrane-associated protein kinase that undergoes autophosphorylation and subsequently transfers the phosphate to PhoP, resulting in the expression of PhoP-activated genes (PAG) and repression of PhoP-repressed genes (PRG). In high periplasmic Mg(2+), acts as a protein phosphatase that dephosphorylates phospho-PhoP, resulting in the repression of PAG and may lead to expression of some PRG. Essential for transcription of spiC inside macrophages by controlling the expression of the two-component regulatory system SsrB/SpiR (SsrA) and Pir at transcriptional and post-transcriptional levels respectively. Promotes expression of the two-component regulatory system PmrA/PmrB via activation of pmrD gene. Is required to attenuate bacterial growth within fibroblast cells and to enhance bacterial resistance to bile in intestinal cells. Negatively regulates prgH, which is required for invasion of epithelial cells. Involved in acid tolerance.
  • 基因功能參考文獻:
    1. These data suggest that when Salmonella experiences mildly acidic pH, the virulence regulator PhoP activation requires the sensor protein PhoQ to detect pH and the Salmonella-specific protein UgtL to amplify the PhoQ response. PMID: 28851823
    2. Salmonella enterica Typhimurium, bearing the acidic pH and divalent cation unresponsive phoQ(W104C-A128C) allele is virulent in mice, indicating that acidic pH and divalent cation sensing by PhoQ are dispensable for virulence. PMID: 26002083
    3. Changes in the periplasmic domain of PhoQ lead to conformational movements in the HAMP domain helices which disrupt interaction between the HAMP and the catalytic domains, thus promoting increased kinase activity. PMID: 26015499
    4. study describes a mechanism whereby the transcriptional activator PhoP elicits expression of dissimilar gene sets when its cognate sensor PhoQ is activated by different signals in the periplasm PMID: 24256574
    5. These data demonstrated that Salmonella Typhimurium PhoQ can sense cationic antimicrobial peptides and CRAMP serves as a putative direct PhoPQ activation signal in the mouse intestine. PMID: 22919691
    6. PhoP/Q regulon enables S. Typhimurium to adapt to intramacrophage stresses other than phagolysosomal fusion. PMID: 21511762
    7. RstB-induction increases the levels of phoP mRNA as well as PhoP protein, while lack of the phoPQ genes abolishes RstB-promoted transcription of the PhoP-regulated genes PMID: 20811812
    8. Enzymatic activity of Salmonella typhimurium PhoQ is directly activated by [host] antimicrobial peptides. Our findings reveal a mechanism by which bacteria sense small innate immune molecules to initiate a transcriptional program that promotes virulence PMID: 16096064
    9. induction of PhoP/PhoQ system results in initial surge of PhoP phosphorylation, occupancy of target promoters by PhoP & transcription of PhoP-activated genes; surge by PhoP's positive feedback loop is necessary to jump-start virulence program PMID: 17158330
    10. analysis of signal-dependent regulation in the PhoP/PhoQ system PMID: 18434315
    11. Mutational analysis and NMR spectroscopy of the periplasmic domains of Salmonella typhimurium PhoQ and Pseudomonas aeruginosa PhoQ indicate distinct mechanisms of binding divalent cation. PMID: 18532985
    12. SlyA has a role as a mediator in signal transduction from the PhoP/PhoQ system to other bacterial two-component systems in Salmonella PMID: 18678876
    13. Data report that the PhoP/PhoQ system regulates different Salmonella genes depending on whether the inducing signal is acidic pH or low Mg(2+). PMID: 19801407

    顯示更多

    收起更多

  • 亞細胞定位:
    Cell inner membrane; Multi-pass membrane protein.
  • 數據庫鏈接:

    KEGG: stm:STM1230

    STRING: 99287.STM1230



主站蜘蛛池模板: 免费国产午夜视频在线观看| 久久久久久久久久久综合日本| 国产真实乱对白精彩久久| 亚洲国产欧美在线人成app| 亚洲爆乳无码精品aaa片蜜桃| 日韩精品人妻系列无码专区 | 夜色福利院在线观看免费| 国产精品亚洲二区在线播放| 欧美成人天天综合在线| 人人添人人澡人人澡人人人人 | 精品久久久无码人妻字幂| 法国啄木乌av片在线播放| 99热亚洲色精品国产88| 天天躁夜夜躁狠狠综合| 强行无套内谢大学生初次| 亚洲精华国产精华精华液网站| 亚洲精品久久国产高清情趣图文 | 精品人伦一区二区三区潘金莲| 久久久综合九色合综国产精品| 日韩av无码一区二区三区不卡毛片| 老子影院午夜伦不卡无码| 国产成a人亚洲精v品无码性色| 亚洲熟妇久久精品| 国产薄丝脚交视频在线观看 | 久久久久无码国产精品不卡| 天堂网www中文在线| 国产在线看片免费视频| 亚洲欧美综合精品成人网站| 老色69久久九九精品高潮 | 337p日本欧洲亚洲大胆色噜噜| 一区二区久久久久草草| 好爽...又高潮了毛片| 香蕉久久精品日日躁夜夜躁夏| 丰满人妻熟妇乱又伦精品软件| 丰满少妇高潮惨叫久久久一| 亚洲综合欧美制服丝袜| 国产偷窥熟妇高潮呻吟| 尤物视频在线观看| 免费永久看黄神器无码软件| 人妻少妇88久久中文字幕| 亚洲人成在线影院|