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  4. Recombinant Salmonella typhimurium Secreted effector protein sseJ (sseJ)

Recombinant Salmonella typhimurium Secreted effector protein sseJ (sseJ)

  • 中文名稱：
    鼠傷寒沙門菌sseJ重組蛋白
  • 貨號：
    CSB-YP861757SXB
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    鼠傷寒沙門菌sseJ重組蛋白
  • 貨號：
    CSB-EP861757SXB
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    鼠傷寒沙門菌sseJ重組蛋白
  • 貨號：
    CSB-EP861757SXB-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    鼠傷寒沙門菌sseJ重組蛋白
  • 貨號：
    CSB-BP861757SXB
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    鼠傷寒沙門菌sseJ重組蛋白
  • 貨號：
    CSB-MP861757SXB
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    sseJ
  • Uniprot No.：
  • 別名：
    sseJ; STM1631; Secreted effector protein SseJ; EC 3.-.-.-; Salmonella-translocated effector J; STE J
  • 種屬：
    Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-408
  • 氨基酸序列
    MPLSVGQGYF TSSISSEKFN AIKESARLPE LSLWEKIKAY FFTTHHAEAL ECIFNLYHHQ ELNLTPVQVR GAYIKLRALA SQGCKEQFII ESQEHADKLI IKDDNGENIL SIEVECHPEA FGLAKEINKS HPKPKNISLG DITRLVFFGD SLSDSLGRMF EKTHHILPSY GQYFGGRFTN GFTWTEFLSS PHFLGKEMLN FAEGGSTSAS YSCFNCIGDF VSNTDRQVAS YTPSHQDLAI FLLGANDYMT LHKDNVIMVV EQQIDDIEKI ISGGVNNVLV MGIPDLSLTP YGKHSDEKRK LKDESIAHNA LLKTNVEELK EKYPQHKICY YETADAFKVI MEAASNIGYD TENPYTHHGY VHVPGAKDPQ LDICPQYVFN DLVHPTQEVH HCFAIMLESF IAHHYSTE
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is required for endosomal tubulation and negatively regulates the formation of Salmonella-induced filaments (Sifs) in epithelial cells. Has both deacylase and esterification activities in vitro, but esterification is probably the dominant activity in host cells. Significantly contributes to cholesterol esterification, which reduces cellular cholesterol in cells and abrogates the ability of SifA to associate with cholesterol and LAMP-1 vesicles.
  • 基因功能參考文獻：
    1. The effects of Salmonella SseJ on kidney epithelial cell microtubules is mediated via its interactions with the RhoA. PMID: 28235057
    2. S. Typhimurium sseJ gene decreases the S. Typhi cytotoxicity in epithelial cells. PMID: 21138562
    3. Data indicating the molecular activity of SseJ suggest that cholesterol and its esterification at the SCV are functionally important for intracellular bacterial survival. PMID: 18333886
    4. The phospholipase A and glycerophospholipid : cholesterol acyltransferase activities of Ssej need to be activated by proteinaceous eukaryotic factors. PMID: 18757801
  • 亞細胞定位：
    Secreted. Host cytoplasm. Note=Secreted via type III secretion system 2 (SPI-2 TTSS), and delivered into the host cytoplasm. Localizes to the Salmonella-containing vacuole (SCV) at early time points following invasion and subsequently traffics away from the SCV along Salmonella-induced filaments (Sifs) in epithelial cells.
  • 蛋白家族：
    'GDSL' lipolytic enzyme family
  • 數據庫鏈接：

    KEGG: stm:STM1631

    STRING: 99287.STM1631



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