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  4. Recombinant Salmonella typhimurium RNA polymerase-binding transcription factor DksA (dksA)

Recombinant Salmonella typhimurium RNA polymerase-binding transcription factor DksA (dksA)

  • 中文名稱：
    鼠傷寒沙門菌dksA重組蛋白
  • 貨號(hào)：
    CSB-YP363090SXB
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    鼠傷寒沙門菌dksA重組蛋白
  • 貨號(hào)：
    CSB-EP363090SXB
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    鼠傷寒沙門菌dksA重組蛋白
  • 貨號(hào)：
    CSB-EP363090SXB-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    鼠傷寒沙門菌dksA重組蛋白
  • 貨號(hào)：
    CSB-BP363090SXB
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    鼠傷寒沙門菌dksA重組蛋白
  • 貨號(hào)：
    CSB-MP363090SXB
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    dksA
  • Uniprot No.：
  • 別名：
    dksA; STM0186RNA polymerase-binding transcription factor DksA
  • 種屬：
    Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-151
  • 氨基酸序列
    MQEGQNRKTS SLSILAIAGV EPYQEKPGEE YMNEAQLSHF KRILEAWRNQ LRDEVDRTVT HMQDEAANFP DPVDRAAQEE EFSLELRNRD RERKLIKKIE KTLKKVEDED FGYCESCGVE IGIRRLEARP TADLCIDCKT LAEIREKQMA G
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Transcription factor that acts by binding directly to the RNA polymerase (RNAP). Required for negative regulation of rRNA expression and positive regulation of several amino acid biosynthesis promoters. Also required for regulation of fis expression.
  • 基因功能參考文獻(xiàn)：
    1. Variations in cysteine and zinc content determine the threshold at which individual DksA proteins sense and respond to reactive species. PMID: 27094335
    2. the data indicate that the ancestral stringent response regulator DksA coordinates various physiological and virulence S. Typhimurium programs and therefore is a key virulence regulator of Salmonella. PMID: 26553464
    3. Transcriptional and Post-Transcriptional Modulation of SPI1 and SPI2 Expression by ppGpp, RpoS and DksA in Salmonella enterica sv Typhimurium PMID: 26039089
    4. Given the conservation of Cys(114) , and neighbouring hydrophobic and charged amino acids in DksA orthologues, microorganisms may use the DksA thiol switch to regulate transcriptional responses to oxidative and nitrosative stress. PMID: 24354846
  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    DksA family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: stm:STM0186

    STRING: 99287.STM0186



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