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  4. Recombinant Salmonella typhimurium Enamine/imine deaminase (ridA)

Recombinant Salmonella typhimurium Enamine/imine deaminase (ridA)

  • 中文名稱：
    鼠傷寒沙門菌ridA重組蛋白
  • 貨號：
    CSB-YP748632SXB
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    鼠傷寒沙門菌ridA重組蛋白
  • 貨號：
    CSB-EP748632SXB
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    鼠傷寒沙門菌ridA重組蛋白
  • 貨號：
    CSB-EP748632SXB-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    鼠傷寒沙門菌ridA重組蛋白
  • 貨號：
    CSB-BP748632SXB
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    鼠傷寒沙門菌ridA重組蛋白
  • 貨號：
    CSB-MP748632SXB
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ridA
  • Uniprot No.：
  • 別名：
    ridA; yjgF; STM4458; 2-iminobutanoate/2-iminopropanoate deaminase; EC 3.5.99.10; Enamine/imine deaminase
  • 種屬：
    Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-128
  • 氨基酸序列
    MSKTIATENA PAAIGPYVQG VDLGSMVITS GQIPVDPKTG AVAEDVSAQA RQSLENVKAI VEAAGLKVGD IVKTTVFVKD LNDFATVNAT YEAFFTEHNA TFPARSCVEV ARLPKDVKIE IEAIAVRR
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Accelerates the release of ammonia from reactive enamine/imine intermediates of the PLP-dependent threonine dehydratase (IlvA) in the low water environment of the cell. It catalyzes the deamination of enamine/imine intermediates to yield 2-ketobutyrate and ammonia. It is required for the detoxification of reactive intermediates of IlvA due to their highly nucleophilic abilities and to avoid they are captured by anthranilate phosphoribosyltransferase (TrpD) to generate PRA, an intermediate in the alternative pyrimidine biosynthetic (APB) pathway. Also required for full activity of IlvE which is involved in the isoleucine biosynthesis. RidA also accelerates the release of pyruvate produced by IlvA from L-serine.
  • 基因功能參考文獻：
    1. Phenotypically, strains of Salmonella enterica that lack RidA accumulated significantly more pyruvate in the growth medium than wild-type strains. PMID: 23815688
    2. Here authors demonstrate the damaging potential of enamines in the absence of RidA (formerly known as YjgF) proteins. PMID: 23386433
    3. Study concludes that the isoleucine requirement of a yjgF mutant on pyruvate is a consequence of the decreased transaminase B (IlvE) activity. PMID: 18296521
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    RutC family
  • 數據庫鏈接：

    KEGG: stm:STM4458

    STRING: 99287.STM4458



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