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  4. Recombinant Saccharomyces cerevisiae pH-response transcription factor pacC/RIM101 (RIM101), partial

Recombinant Saccharomyces cerevisiae pH-response transcription factor pacC/RIM101 (RIM101), partial

  • 中文名稱：
    釀酒酵母RIM101重組蛋白
  • 貨號：
    CSB-YP341415SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母RIM101重組蛋白
  • 貨號：
    CSB-EP341415SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母RIM101重組蛋白
  • 貨號：
    CSB-EP341415SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母RIM101重組蛋白
  • 貨號：
    CSB-BP341415SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母RIM101重組蛋白
  • 貨號：
    CSB-MP341415SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    RIM101
  • Uniprot No.：
  • 別名：
    RIM101; RIM1; YHL027W; pH-response transcription factor pacC/RIM101; Regulator of IME2 protein 1; pH-response regulator protein RIM101
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Transcription factor that mediates regulation of both acid- and alkaline-expressed genes in response to ambient pH. At alkaline ambient pH, activates transcription of alkaline-expressed genes (including RIM101 itself), mainly by repressing transcriptional repressors of those genes, and represses transcription of acid-expressed genes. Required for meiosis, sporulation and invasive growth.
  • 基因功能參考文獻：
    1. These results suggest that the Rim101 pathway is important for the adaptation to ER stress by compensating for alterations in plasma membrane lipid asymmetry induced by ER stress. PMID: 27803246
    2. We conclude that summation operator1278b may harbor a variant(s) that buffers against widespread transcriptional dysregulation upon introduction of a non-native RIM101 allele, emphasizing the importance of accounting for genetic background . PMID: 26751950
    3. A role of Rim101 in modulation of vacuolar acidity necessary for selenite detoxification is discovered. PMID: 25239548
    4. Results found that activity of the Rsp5 ubiquitin ligase was required for recruiting the downstream molecules to the plasma membrane, suggesting that ubiquitination mediates Rim101 signaling at the plasma membrane. PMID: 25002535
    5. Pho85 regulates the nuclear accumulation of active Rim101, possibly via phosphorylation PMID: 20382759
    6. Rim101 pathway mediates colony patterning by responding to cell-to-cell pH signals. PMID: 20038633
    7. RIM101 pathway contributes to cell wall assembly and its function becomes essential in the absence of SIt2. PMID: 16524906
    8. RIM101 expression is shown to be required to counteract propionic acid-induced cytosolic acidification and for proper vacuolar acidification and cell wall structure. PMID: 19220866
    9. The double mutant slt2Delta rim101Delta displays altered chitin rings, with a significant reduction in the levels of chitin. PMID: 19633265

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  • 亞細胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    PacC/RIM101 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YHL027W

    STRING: 4932.YHL027W



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