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Recombinant Saccharomyces cerevisiae mRNA-decapping enzyme subunit 2 (DCP2), partial

  • 中文名稱:
    Recombinant Saccharomyces cerevisiae mRNA-decapping enzyme subunit 2(DCP2) ,partial
  • 貨號:
    CSB-YP347214SVG
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    Recombinant Saccharomyces cerevisiae mRNA-decapping enzyme subunit 2(DCP2) ,partial
  • 貨號:
    CSB-EP347214SVG
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    Recombinant Saccharomyces cerevisiae mRNA-decapping enzyme subunit 2(DCP2) ,partial
  • 貨號:
    CSB-EP347214SVG-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    Recombinant Saccharomyces cerevisiae mRNA-decapping enzyme subunit 2(DCP2) ,partial
  • 貨號:
    CSB-BP347214SVG
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    Recombinant Saccharomyces cerevisiae mRNA-decapping enzyme subunit 2(DCP2) ,partial
  • 貨號:
    CSB-MP347214SVG
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    DCP2
  • Uniprot No.:
  • 別名:
    DCP2; PSU1; YNL118C; N1917m7GpppN-mRNA hydrolase; EC 3.6.1.62; Protein PSU1; mRNA-decapping enzyme subunit 2
  • 種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度:
    Partial
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Catalytic component of the decapping complex necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Decapping is the major pathway of mRNA degradation in yeast and occurs through deadenylation, decapping and subsequent 5' to 3' exonucleolytic decay of the transcript body. Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts.
  • 基因功能參考文獻:
    1. This recruitment requires Hrr25 kinase activity and the Dcp2 decapping enzyme, a core constituent of these RNP granules. Interestingly, the data indicate that this localization sequesters active Hrr25 away from the remainder of the cytoplasm and thereby shields this enzyme from the degradation machinery during these periods of stress. PMID: 27182950
    2. Results show that C-terminal domain of Dcp2 includes a set of conserved negative and positive regulatory elements reveal a pivotal role for the Dcp2 in mRNA decapping and lead to a new model for decapping enzyme regulation in vivo. PMID: 26184073
    3. Local active site conformational changes are important for Dcp2 mRNA decapping activity. PMID: 23911090
    4. show using NMR that cap binds conserved residues on both the catalytic and regulatory domains of Dcp2. PMID: 20711189
    5. Ste20 has an unexpected role in the modulation of mRNA decay and translation in Saccharomyces cerevisiae. Phosphorylation of Dcp2 by Ste20 is an important control point for mRNA decapping. PMID: 20513766
    6. These results raise a model for Dcp2 recruitment to specific mRNAs where regions outside the catalytic core promote the formation of different complexes involved in mRNA decapping. PMID: 20086104
    7. To understand regulation of Dcp2 by ligand interactions, we have assigned the backbone and sidechain methyl Ile (delta1), Leu and Val chemical shifts of the catalytic domain. PMID: 19636815

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  • 亞細胞定位:
    Cytoplasm, P-body.
  • 蛋白家族:
    Nudix hydrolase family, DCP2 subfamily
  • 數據庫鏈接:

    KEGG: sce:YNL118C

    STRING: 4932.YNL118C



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