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Recombinant Saccharomyces cerevisiae Zinc-responsive transcriptional regulator ZAP1 (ZAP1), partial

  • 中文名稱:
    釀酒酵母ZAP1重組蛋白
  • 貨號:
    CSB-YP342851SVG
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    釀酒酵母ZAP1重組蛋白
  • 貨號:
    CSB-EP342851SVG
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    釀酒酵母ZAP1重組蛋白
  • 貨號:
    CSB-EP342851SVG-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    釀酒酵母ZAP1重組蛋白
  • 貨號:
    CSB-BP342851SVG
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    釀酒酵母ZAP1重組蛋白
  • 貨號:
    CSB-MP342851SVG
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    ZAP1
  • Uniprot No.:
  • 別名:
    ZAP1; YJL056C; J1145; Zinc-responsive transcriptional regulator ZAP1
  • 種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度:
    Partial
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Transcription regulator controlling zinc-responsive gene expression. Binds to zinc-responsive elements (ZREs) (consensus sequence 5'-ACCYYNAAGGT-3') in the promoter of target genes. Recruits SWI/SNF, SAGA, and Mediator complexes as coactivators in a zinc-responsive manner. Involved in zinc ion homeostasis by zinc-responsive transcriptional regulation of the zinc uptake system genes ZTR1 and ZTR2. Positively regulates ETR1 expression, affecting mitochondrial function.
  • 基因功能參考文獻:
    1. In this study,the authors performed the first phylogenetic classification of shochu strains (Saccharomyces cerevisiae strains) using the nucleotide sequences of genes that reflect genome-level phylogeny. The single-nucleotide polymorphisms information identified in the present study is expected to be useful for distinguishing shochu yeast strains as part of quality control at shochu breweries PMID: 28703391
    2. Results suggest that the Zap1 regulon includes ZRG17; Zap1-directed transcriptional regulation has biological importance in maintaining ER function and zinc homeostasis. PMID: 21250939
    3. Roles of two activation domains in Zap1 in the response to zinc deficiency in Saccharomyces cerevisiae. PMID: 21177862
    4. AD1 (activation domain) residues 332-402 is critical for control of gene expression in response to zinc PMID: 16045625
    5. results describe the solution structure of two zinc fingers (zf1-2(CA3)) derived from a zinc-responsive domain of Zap1 (zf1-2) PMID: 16483601
    6. results indicate that the kinetic lability of ZF1/2 in vivo is a key component of Zap1 zinc responsiveness PMID: 16720702
    7. During zinc deficiency, Zap1 mediates the repression of two of the most abundant zinc-requiring enzymes. PMID: 17139254
    8. through the differential regulation of its target genes, Zap1 prioritizes mechanisms of zinc homeostasis and adaptive responses to zinc deficiency PMID: 18673560

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  • 亞細胞定位:
    Nucleus.
  • 數據庫鏈接:

    KEGG: sce:YJL056C

    STRING: 4932.YJL056C



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