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  4. Recombinant Saccharomyces cerevisiae Vacuolar membrane protein PEP5 (PEP5), partial

Recombinant Saccharomyces cerevisiae Vacuolar membrane protein PEP5 (PEP5), partial

  • 中文名稱：
    釀酒酵母PEP5重組蛋白
  • 貨號：
    CSB-YP321027SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母PEP5重組蛋白
  • 貨號：
    CSB-EP321027SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母PEP5重組蛋白
  • 貨號：
    CSB-EP321027SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母PEP5重組蛋白
  • 貨號：
    CSB-BP321027SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母PEP5重組蛋白
  • 貨號：
    CSB-MP321027SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    PEP5
  • Uniprot No.：
  • 別名：
    PEP5; END1; VAM1; VPL9; VPS11; VPT11; YMR231W; YM9959.13; E3 ubiquitin-protein ligase PEP5; EC 2.3.2.27; Carboxypeptidase Y-deficient protein 5; Histone E3 ligase PEP5; RING-type E3 ubiquitin transferase PEP5; Vacuolar biogenesis protein END1; Vacuolar morphogenesis protein 1; Vacuolar protein sorting-associated protein 11; Vacuolar protein-targeting protein 11
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Required for vacuolar biogenesis and for trafficking of hydrolase precursors to the vacuole. Mediates transport at the vacuolar membrane where it may be responsible for tethering transport vesicles on the target membranes. It is required for gluconeogenic growth of yeast. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion. Acts as component of the CORVET complex that is required for transport between endosome and vacuole. CORVET is an effector for the endosomal Rab GTPase VPS21. Probable ubiquitin-protein ligase involved in the degradation-related ubiquitination of histones. Contributes to the post-translational regulation of histone protein levels by polyubiquitination of excess histones for subsequent degradation.
  • 基因功能參考文獻：
    1. The results highlight the importance of posttranslational histone regulatory mechanisms that employ multiple E3 ubiquitin ligases such as Pep5 to ensure excess histone degradation and thus contribute to the maintenance of genomic stability. PMID: 22570702
    2. The C-terminal domain of Vps11 is a key integrator of CORVET (class C core vacuole/endosome tethering complex) assembly, Rab signaling, and endosomal and lysosomal traffic. PMID: 21325627
  • 亞細胞定位：
    Vacuole membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    VPS11 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YMR231W

    STRING: 4932.YMR231W



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