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Recombinant Saccharomyces cerevisiae Transcriptional activator HAC1 (HAC1)

  • 中文名稱:
    釀酒酵母HAC1重組蛋白
  • 貨號:
    CSB-YP328265SVG
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    釀酒酵母HAC1重組蛋白
  • 貨號:
    CSB-EP328265SVG
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    釀酒酵母HAC1重組蛋白
  • 貨號:
    CSB-EP328265SVG-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    釀酒酵母HAC1重組蛋白
  • 貨號:
    CSB-BP328265SVG
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    釀酒酵母HAC1重組蛋白
  • 貨號:
    CSB-MP328265SVG
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    HAC1
  • Uniprot No.:
  • 別名:
    HAC1; ERN4; IRE15; IRE2; YFL031WTranscriptional activator HAC1
  • 種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-238
  • 氨基酸序列
    MEMTDFELTS NSQSNLAIPT NFKSTLPPRK RAKTKEEKEQ RRIERILRNR RAAHQSREKK RLHLQYLERK CSLLENLLNS VNLEKLADHE DALTCSHDAF VASLDEYRDF QSTRGASLDT RASSHSSSDT FTPSPLNCTM EPATLSPKSM RDSASDQETS WELQMFKTEN VPESTTLPAV DNNNLFDAVA SPLADPLCDD IAGNSLPFDN SIDLDNWRNP EAQSGLNSFE LNDFFITS
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Transcriptional activator involved in the unfolded protein response (UPR) pathway. Recognizes and binds to the UPR element (UPRE) in the promoter of UPR-regulated genes such as KAR2, PDI1, EUG1 and FKB2. Increases the synthesis of endoplasmic reticulum-resident proteins required for protein folding as well as components of the secretory pathway.
  • 基因功能參考文獻:
    1. Here, the authors show that a combination of inhibited translation initiation and accelerated protein degradation-both dependent on the intron-prevents the accumulation of Hac1 protein when the unfolded protein response is inactive. PMID: 27692069
    2. Hac1p restored the Golgi localization of Arl1p and Imh1p inIRE1-deleted cells. Ire1p-Hac1p axis regulated Syt1p phosphorylation, which enhances Arl1p activation, recruitment of Imh1p to the Golgi, and Syt1p interaction with Arl1p. PMID: 26966233
    3. Results indicate that the 5'-untranslated region (5' UTR)-intron interaction represses translation initiation on the unspliced Hac1 transcription factor mRNA. PMID: 26175153
    4. Kin2 has a role in regulating HAC1 mRNA translocation, splicing, and translation PMID: 25348718
    5. Our data suggest a complex mutual cross talk between the Hac1- and Gcn4-controlled networks. PMID: 23794510
    6. Expression of rat Bax induces the unfolded protein response by inducing HAC1 mRNA splicing in Saccharomyces cerevisiae. PMID: 22903551
    7. the splicing and the release of translational attenuation of HAC1 mRNA are separable steps PMID: 20844078
    8. transcription of HAC1 is regulated through a pathway involving IRE1 PMID: 15314654
    9. Gcn4p, Hac1p, and Gcn2p regulate targets of the unfolded protein response PMID: 15314660
    10. Hac1p harbors a functional PEST degron and t degradation of Hac1p by the proteasome involves the E2 ubiquitin-conjugating enzyme Ubc3/Cdc34p and the SCF(Cdc4) E3 complex. PMID: 17108329
    11. Ricin inhibits activation of the unfolded protein response by preventing splicing of the HAC1 mRNA PMID: 18180297

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  • 亞細胞定位:
    Nucleus.
  • 蛋白家族:
    BZIP family
  • 數據庫鏈接:

    KEGG: sce:YFL031W

    STRING: 4932.YFL031W



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