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  4. Recombinant Saccharomyces cerevisiae Transcription elongation factor SPT6 (SPT6), partial

Recombinant Saccharomyces cerevisiae Transcription elongation factor SPT6 (SPT6), partial

  • 中文名稱：
    釀酒酵母SPT6重組蛋白
  • 貨號(hào)：
    CSB-YP022960SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母SPT6重組蛋白
  • 貨號(hào)：
    CSB-EP022960SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母SPT6重組蛋白
  • 貨號(hào)：
    CSB-EP022960SVG-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母SPT6重組蛋白
  • 貨號(hào)：
    CSB-BP022960SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母SPT6重組蛋白
  • 貨號(hào)：
    CSB-MP022960SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    SPT6
  • Uniprot No.：
  • 別名：
    SPT6; CRE2; SSN20; YGR116W; G6169; Transcription elongation factor SPT6; Chromatin elongation factor SPT6
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Plays a role in maintenance of chromatin structure during RNA polymerase II transcription elongation thereby repressing transcription initiation from cryptic promoters. Mediates the reassembly of nucleosomes onto the promoters of at least a selected set of genes during repression; the nucleosome reassembly is essential for transcriptional repression. Essential for viability.
  • 基因功能參考文獻(xiàn)：
    1. Results provide evidence that both FACT and Spt6 are important for transcription globally and may participate during different stages of transcription. PMID: 29695490
    2. Interactions between SPT6 and RNA Polymerase II PMID: 27261007
    3. Results demonstrate that a fine-tuning of the Spt16 component of FACT by UPS is essential for proper chromatin reassembly and efficient transcriptional elongation, thus revealing a novel ubiquitin proteasome system regulation of FACT with physiological relevance in gene expression. PMID: 27044865
    4. Histone chaperones FACT and Spt6 are important for appropriate H2AZ histone localization and for the functioning of RNA polymerase II. PMID: 26990181
    5. Data show that the histone chaperones POB3 protein FACT and chromatin-remodeling histone chaperone Spt6 prevent widespread accumulation of histone H2A.Z. PMID: 25959393
    6. Spt6 is involved in either recruiting the Pol I-Rrn3 complex to the rDNA or stabilizing the preinitiation complex. The findings presented here identify an unexpected, essential role for Spt6 in synthesis of rRNA. PMID: 25918242
    7. Data suggest that phosphorylation of the Pol II C-terminal domain on Tyr1 promotes the association of Spt6 with the 3' ends of transcribed genes, independently of Ser2 phosphorylation. PMID: 25182531
    8. A report on the mechanism involving Spt6 and Ctk1 in the regulation and maintenance of RNA polymerase II Ser2 C-terminal domain phosphorylation during transcription elongation. PMID: 24163256
    9. Spt6 may use the tandem SH2 domain module to sense the phosphorylation level of RNAPII CTD. PMID: 21676864
    10. these findings provide a structural foundation for understanding how Spt6 encodes several distinct functions within a single polypeptide chain. PMID: 21419780
    11. results provide new evidence for a connection between the transcription elongation factor Spt6 and RNA 3'-end formation in vivo PMID: 15531585
    12. Results identify Spt6 as the factor that mediates nucleosome reassembly onto the PHO5 and other promoters during transcriptional repression. PMID: 16455495
    13. These findings link Spn1 functions to the transition from an inactive to an actively transcribing RNAPII complex at a postrecruitment-regulated promoter. PMID: 18086892
    14. The C-terminal region of Set2, in conjunction with the phosphorylated C-terminal domain of RNAPII, influences the KMTase activity to promote methylation of histone H3 at lysine 36 during transcription elongation. PMID: 18541663
    15. The SH2 domain is required for normal expression of a subset of yeast genes, and is consistent with multiple functions of Spt6 in chromatin transcription. PMID: 19371747

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  • 亞細(xì)胞定位：
    Nucleus. Note=Colocalizes with RNA polymerase II on chromatin. Recruited to the active transcribed loci.
  • 蛋白家族：
    SPT6 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YGR116W

    STRING: 4932.YGR116W



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