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  4. Recombinant Saccharomyces cerevisiae Transcription elongation factor SPT5 (SPT5), partial

Recombinant Saccharomyces cerevisiae Transcription elongation factor SPT5 (SPT5), partial

  • 中文名稱：
    釀酒酵母SPT5重組蛋白
  • 貨號：
    CSB-YP022959SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母SPT5重組蛋白
  • 貨號：
    CSB-EP022959SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母SPT5重組蛋白
  • 貨號：
    CSB-EP022959SVG-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母SPT5重組蛋白
  • 貨號：
    CSB-BP022959SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母SPT5重組蛋白
  • 貨號：
    CSB-MP022959SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    SPT5
  • Uniprot No.：
  • 別名：
    SPT5; YML010W; YM9571.08; Transcription elongation factor SPT5; Chromatin elongation factor SPT5
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    The SPT4-SPT5 complex mediates both activation and inhibition of transcription elongation, and plays a role in pre-mRNA processing. This complex seems to be important for the stability of the RNA polymerase II elongation machinery on the chromatin template but not for the inherent ability of this machinery to translocate down the gene.
  • 基因功能參考文獻：
    1. Interactions between SPT5 and RNA Polymerase II PMID: 27261007
    2. Spt5 is crucial for a normal rate of RNA synthesis and distribution of RNAPII over transcription units. PMID: 28366642
    3. High-resolution crystallographic results demonstrating that distinct structures are formed by the first through third KOW domains of Saccharomyces cerevisiae Spt5. PMID: 26217010
    4. The findings suggest that Spt5 is a key coordinator for holding the RNA polymerase II complex in a closed conformation that is highly competent for transcription elongation but repressive to transcription coupled DNA repair. PMID: 24813444
    5. Chromatin immunoprecipitation (ChIP) revealed that the Spt5 C-terminal region is required for normal recruitment of pre-mRNA cleavage factor I (CFI) to the 3' ends of Saccharomyces cerevisiae genes. PMID: 22290438
    6. Spt5 is recruited to the rDNA early in transcription and propose that it plays an important role in ribosomal RNA synthesis through direct binding to the Pol I complex. PMID: 21467036
    7. Spt5p plays both positive and negative roles in transcription by Pol I. PMID: 21467039
    8. suppression of spt5 can result from loss of histone H3 lysines 4 or 36 methylation, or reduced recruitment of Chd1 or the Rpd3S complex PMID: 19948887
    9. Data show that Bur1 phosphorylates the Spt5 C-terminal repeat domain (CTD) both in vivo and in isolated elongation complexes in vitro. PMID: 19581288

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  • 亞細胞定位：
    Nucleus. Mitochondrion.
  • 蛋白家族：
    SPT5 family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YML010W

    STRING: 4932.YML010W



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