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  4. Recombinant Saccharomyces cerevisiae TATA-binding protein-associated factor MOT1 (MOT1), partial

Recombinant Saccharomyces cerevisiae TATA-binding protein-associated factor MOT1 (MOT1), partial

  • 中文名稱：
    釀酒酵母MOT1重組蛋白
  • 貨號：
    CSB-YP329158SVG
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母MOT1重組蛋白
  • 貨號：
    CSB-EP329158SVG
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母MOT1重組蛋白
  • 貨號：
    CSB-EP329158SVG-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母MOT1重組蛋白
  • 貨號：
    CSB-BP329158SVG
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母MOT1重組蛋白
  • 貨號：
    CSB-MP329158SVG
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MOT1
  • Uniprot No.：
  • 別名：
    MOT1; YPL082C; LPF4C; TATA-binding protein-associated factor MOT1; TBP-associated factor MOT1; EC 3.6.4.-; Modifier of transcription 1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box via its C-terminal ATPase activity. Both transcription activation and repression require its ATPase activity.
  • 基因功能參考文獻：
    1. these results suggest that TBP-DNA affinity as well as other aspects of promoter sequence influence Mot1 function in vivo. PMID: 27255709
    2. The results stress the importance of the cooperation of Mot1p/NC2 with remodelers in maintaining a repressive environment for intragenic transcription. PMID: 24459134
    3. The action of Mot1 is required to clear TBP from intrinsically preferred (TATA-containing) binding sites, ensuring sufficient soluble TBP to bind intrinsically disfavored (TATA-less) sites. PMID: 24144978
    4. In the absence of ATP, ternary complex stability arises from both Mot1-DNA and Mot1-TBP interactions. PMID: 22298788
    5. findings suggest that the four conserved regions of Mot1 are essential for viability and required for proper regulation of most Mot1-regulated genes; loss of the ATPase domain, however, imparts some unexpected regulation on certain genes PMID: 21444714
    6. Mot1 plays a broad role in establishing the precision and efficiency of RNA synthesis. PMID: 20855454
    7. MOT1 might have a role in vivo as a transcriptional activator in the small ubiquitin-related modifier (SUMO) protein pathway. PMID: 16387868
    8. A novel ATP-independent TBPc displacement activity for Mot1 is reported PMID: 16541100
    9. These results support a model in which Mot1 affects the rate and efficiency of rRNA synthesis by both direct and indirect mechanisms, with resulting effects on transcription activation and the coupling of rRNA synthesis to processing. PMID: 17296733
    10. Mot1p interacts with a range of transcriptional co-regulators as well as proteins involved in chromatin remodeling. PMID: 18596064
    11. Mot1-mediated dissociation of TBP (or TBP-containing complexes) from chromatin can explain the Mot1 activation mechanism at some promoters. PMID: 19098311
    12. These results therefore demonstrate that Mot1 is an in vivo SUMO-targeted ubiquitin ligase target in yeast and suggest a role for SUMO-targeted degradation in protein quality control. PMID: 19139279

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  • 亞細胞定位：
    Mitochondrion. Nucleus. Note=Localized on chromatin. Specifically localized to the promoters of the genes it regulates.
  • 蛋白家族：
    SNF2/RAD54 helicase family
  • 數據庫鏈接：

    KEGG: sce:YPL082C

    STRING: 4932.YPL082C



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