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  4. Recombinant Saccharomyces cerevisiae Single-strand annealing weakened protein 1 (SAW1)

Recombinant Saccharomyces cerevisiae Single-strand annealing weakened protein 1 (SAW1)

  • 中文名稱：
    釀酒酵母SAW1重組蛋白
  • 貨號：
    CSB-YP340145SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母SAW1重組蛋白
  • 貨號：
    CSB-EP340145SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母SAW1重組蛋白
  • 貨號：
    CSB-EP340145SVG-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母SAW1重組蛋白
  • 貨號：
    CSB-BP340145SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母SAW1重組蛋白
  • 貨號：
    CSB-MP340145SVG
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    SAW1
  • Uniprot No.：
  • 別名：
    SAW1; YAL027W; Single-strand annealing weakened protein 1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-261
  • 氨基酸序列
    MAPSIATVKI ARDMVLPLRI FVNRKQILQT NDKTSNKSNA TIFEAPLLSN NSIICLKSPN TRIYLSQQDK KNLCDEIKED LLLIVYELAS PEIISSVLSK IRVGHSTDFQ INVLPKLFAG ADTDNAVTSH IQSVTRLAKF KYKLHYKHKW ELDIFINSIK KIANLRHYLM FQTLTLNGFS LNAGPKTLLA RKIEKQPQVP NLLIENGDAD ALDTPVEEDI KPVIEFMYKP VINLGEIIDV HVLHRPRRHK VRTQSKQPQE E
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Catalyzes 3'-non-homologous tail removal of RAD1/RAD10-dependent single-strand annealing recombination intermediates. Plays a key role in targeting RAD1/RAD10 complex to 3'-flap cleavage substrate in recombination. Also contributes to the integrity of ribosomal DNA arrays.
  • 基因功能參考文獻：
    1. the combination of dynamic light scattering and differential scanning fluorimetry techniques to optimize the stability and homogeneity of recombinant Saw1. The protein expression and purification conditions identified in this study allow for higher recovery of soluble Saw1 and enable the biochemical characterization of the protein. PMID: 28263853
    2. results support a model in which Saw1 and Rad1-Rad10 are recruited as a complex to short and long flaps in all phases of cell cycle, but that Saw1 is only required for recruitment of Rad1-Rad10 to DSBs when long flaps are formed in G1 PMID: 26699908
    3. Saw1 and its sumoylation suggest that Saw1 is a multifunctional scaffold that can facilitate diverse types of DNA repair through its modification and nuclease interactions. PMID: 25263559
    4. Saw1 is required for Synthesis Dependent Strand Annealing double-strand break repair in S. cerevisiae. PMID: 24565838
    5. Saw1 plays a role in Rad1/Rad10 complex assembly at recombination intermediates in budding yeast. PMID: 23299942
    6. Data suggest that Saw1 targets Rad1/Rad10 to Rad52-coated recombination intermediates. PMID: 18471978

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  • 亞細胞定位：
    Nucleus.
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YAL027W

    STRING: 4932.YAL027W



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