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  4. Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase RIO1 (RIO1)

Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase RIO1 (RIO1)

  • 中文名稱：
    釀酒酵母RIO1重組蛋白
  • 貨號：
    CSB-YP613205SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母RIO1重組蛋白
  • 貨號：
    CSB-EP613205SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母RIO1重組蛋白
  • 貨號：
    CSB-EP613205SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母RIO1重組蛋白
  • 貨號：
    CSB-BP613205SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母RIO1重組蛋白
  • 貨號：
    CSB-MP613205SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    RIO1
  • Uniprot No.：
  • 別名：
    RIO1; RRP10; YOR119C; O3266; YOR3266C; Serine/threonine-protein kinase RIO1; EC 2.7.11.1; EC 3.6.3.-; Ribosomal RNA-processing protein 10
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-484
  • 氨基酸序列
    MSLEDKFDSL SVSQGASDHI NNQLLEKYSH KIKTDELSFS RAKTSKDKAN RATVENVLDP RTMRFLKSMV TRGVIADLNG CLSTGKEANV YHAFAGTGKA PVIDEETGQY EVLETDGSRA EYAIKIYKTS ILVFKDRERY VDGEFRFRNS RSQHNPRKMI KIWAEKEFRN LKRIYQSGVI PAPKPIEVKN NVLVMEFLSR GNGFASPKLK DYPYKNRDEI FHYYHTMVAY MRLLYQVCRL VHADLSEYNT IVHDDKLYMI DVSQSVEPEH PMSLDFLRMD IKNVNLYFEK MGISIFPERV IFQFVISETL EKFKGDYNNI SALVAYIASN LPIKSTEQDE AEDEIFRSLH LVRSLGGLEE RDFDRYTDGK FDLLKSLIAH DNERNFAASE QFEFDNADHE CSSGTEEFSD DEEDGSSGSE EDDEEEGEYY DDDEPKVLKG KKHEDKDLKK LRKQEAKDAK REKRKTKVKK HIKKKLVKKT KSKK
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Required for the final endonucleolytic cleavage at site D converting 20S pre-rRNA into the mature 18S rRNA. Required for the final steps of cytoplasmic maturation of the 40S ribosomal subunit. The association with the very late 40S subunit intermediate seems to follow RIO2 association with precursors of the 40S subunit and may involve a translation-like checkpoint point cycle preceeding the binding to the 60S ribosomal subunit. Despite the protein kinase domain is proposed to act predominantly as an ATPase. The catalytic activity regulates its dynamic association with the 40S subunit. Has a role in the cell cycle where it is required for entrance into S-phase and in the control of the onset of anaphase. Appears to also be involved in the maintenance of chromosome stability and correct mitotic segregation.
  • 基因功能參考文獻：
    1. The study indicates that lack of Rio1p allows premature entry of pre-40S particles in the translation process. PMID: 28977579
    2. Nuclear Rio1 promotes rDNA array stability and segregation. PMID: 25851096
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Protein kinase superfamily, RIO-type Ser/Thr kinase family
  • 數據庫鏈接：

    KEGG: sce:YOR119C

    STRING: 4932.YOR119C



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