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Recombinant Saccharomyces cerevisiae SWI5-dependent HO expression protein 2 (SHE2)

  • 中文名稱:
    釀酒酵母SHE2重組蛋白
  • 貨號:
    CSB-YP330529SVG
  • 規(guī)格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    釀酒酵母SHE2重組蛋白
  • 貨號:
    CSB-EP330529SVG
  • 規(guī)格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    釀酒酵母SHE2重組蛋白
  • 貨號:
    CSB-EP330529SVG-B
  • 規(guī)格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    釀酒酵母SHE2重組蛋白
  • 貨號:
    CSB-BP330529SVG
  • 規(guī)格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    釀酒酵母SHE2重組蛋白
  • 貨號:
    CSB-MP330529SVG
  • 規(guī)格:
  • 來源:
    Mammalian cell
  • 其他:

產(chǎn)品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    SHE2
  • Uniprot No.:
  • 別名:
    SHE2; YKL130C; SWI5-dependent HO expression protein 2
  • 種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度:
    full length protein
  • 表達區(qū)域:
    1-246
  • 氨基酸序列
    MSKDKDIKVT PGTCELVEQI LALLSRYLSS YIHVLNKFIS HLRRVATLRF ERTTLIKFVK KLRFYNDCVL SYNASEFINE GKNELDPEAD SFDKVILPIA SMFVKCVETF DLLNYYLTQS LQKEILSKTL NEDLTLTAES ILAIDDTYNH FVKFSQWMIE SLRIGSNLLD LEVVQFAIKC ADEDGTNIGE TDNIFLQEIL PVNSEEEFQT LSAAWHSILD GKLSALDEEF DVVATKWHDK FGKLKN
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能:
    RNA-binding protein that binds specific mRNAs including the ASH1 mRNA, coding for a repressor of the HO endonuclease. Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Recruits the MYO4-SHE3 complex to the ASH1 mRNA. Recruits also LOC1 and PUF6 to ASH1 mRNA, which are required for translational repression of this mRNA.
  • 基因功能參考文獻:
    1. Combining RNA- and membrane-binding features makes She2p an ideal coordinator of ER tubule and mRNA cotransport. PMID: 24056370
    2. The binding site for She3p was mapped to a single alpha helix that protrudes at right angles from She2p. PMID: 22084309
    3. She2p is recruited cotranscriptionally to the nascent bud-localized ASH1, IST2, and EAR1 mRNA PMID: 20713510
    4. She2p is a member of a previously unknown class of nucleic acid binding proteins, composed of a single globular domain with a five alpha helix bundle that forms a symmetric homodimer. PMID: 15537539
    5. mRNA localization involves association of She2p and mRNPs with endoplasmic reticulum tubules and myosin-dependent cotransport of tubules and localized mRNPs. PMID: 16890529
    6. Results indicate that the nucleolar transit of RNA-binding proteins such as She2 is necessary for the correct assembly of translationally silenced localizing messenger ribonucleoproteins. PMID: 18566598
    7. finding on She2p tetramerization resolves previously raised questions on complex formation and mRNP function. PMID: 19710186

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  • 亞細胞定位:
    Cytoplasm. Nucleus.
  • 蛋白家族:
    SHE2 family
  • 數(shù)據(jù)庫鏈接:

    KEGG: sce:YKL130C

    STRING: 4932.YKL130C



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