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  4. Recombinant Saccharomyces cerevisiae SCF E3 ubiquitin ligase complex F-box protein GRR1 (GRR1), partial

Recombinant Saccharomyces cerevisiae SCF E3 ubiquitin ligase complex F-box protein GRR1 (GRR1), partial

  • 中文名稱：
    釀酒酵母GRR1重組蛋白
  • 貨號：
    CSB-YP333026SVG
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母GRR1重組蛋白
  • 貨號：
    CSB-EP333026SVG
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母GRR1重組蛋白
  • 貨號：
    CSB-EP333026SVG-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母GRR1重組蛋白
  • 貨號：
    CSB-BP333026SVG
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母GRR1重組蛋白
  • 貨號：
    CSB-MP333026SVG
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    GRR1
  • Uniprot No.：
  • 別名：
    GRR1; CAT80; COT2; YJR090C; J1885SCF E3 ubiquitin ligase complex F-box protein GRR1; F-box and leucine-rich repeat protein GRR1; F-box/LRR-repeat protein GRR1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and directs ubiquitination of phosphorylated CLN1, CLN2 and GIC2. Probably constitutes the primary response element required for the generation or interpretation of the signal that induces glucose repression.
  • 基因功能參考文獻：
    1. We find that Ndd1 turnover in metaphase requires Cdk1 activity and the ubiquitin ligase SCF(Grr1). PMID: 25894965
    2. Data indicate that Npr2, a homolog of human NPRL2, is a phosphorylation-dependent target of the SCF(Grr1) E3 ubiquitin ligase that plays a role in cell growth on some nitrogen sources. PMID: 20154027
    3. Data suggest that casein kinase I plays a role in fungal nutritional signaling pathways by phosphorylating pathway components and targeting them for ubiquitination by SCFGrr1. PMID: 15456873
    4. Grr1p plays an important role in the regulatory pathways that ultimately lead to transcriptional regulation by each of the components Mig1p and Rgt1p. PMID: 15556081
    5. Gis4 may explain the glucose repression defects in carbon source metabolism for the grr1 mutants PMID: 15890364
    6. required for pheromone sensitivity PMID: 15942932
    7. A proteomic screen reveals SCFGrr1 targets that regulate the glycolytic-gluconeogenic switch. PMID: 17828247

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  • 亞細胞定位：
    Membrane. Note=Associated with the particulate fraction. Probably forms a complex by protein-protein interactions via its leucine-rich segment.
  • 數據庫鏈接：

    KEGG: sce:YJR090C

    STRING: 4932.YJR090C



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