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Recombinant Saccharomyces cerevisiae RNA polymerase II subunit A C-terminal domain phosphatase SSU72 (SSU72)

  • 中文名稱:
    釀酒酵母SSU72重組蛋白
  • 貨號:
    CSB-YP022729SVG
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    釀酒酵母SSU72重組蛋白
  • 貨號:
    CSB-EP022729SVG
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    釀酒酵母SSU72重組蛋白
  • 貨號:
    CSB-EP022729SVG-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    釀酒酵母SSU72重組蛋白
  • 貨號:
    CSB-BP022729SVG
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    釀酒酵母SSU72重組蛋白
  • 貨號:
    CSB-MP022729SVG
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    SSU72
  • Uniprot No.:
  • 別名:
    SSU72; YNL222W; N1279; RNA polymerase II subunit A C-terminal domain phosphatase SSU72; CTD phosphatase SSU72; EC 3.1.3.16; Suppressor of SUA7 protein 2
  • 種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-206
  • 氨基酸序列
    MPSHRNSNLK FCTVCASNNN RSMESHKVLQ EAGYNVSSYG TGSAVRLPGL SIDKPNVYSF GTPYNDIYND LLSQSADRYK SNGLLQMLDR NRRLKKAPEK WQESTKVFDF VFTCEERCFD AVCEDLMNRG GKLNKIVHVI NVDIKDDDEN AKIGSKAILE LADMLNDKIE QCEKDDIPFE DCIMDILTEW QSSHSQLPSL YAPSYY
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. Component of the APT complex, which may be involved in polyadenylation-independent transcript 3'-end formation. SSU72 is required for 3'-end formation of snoRNAs.; Processively dephosphorylates Ser-5 of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1).
  • 基因功能參考文獻:
    1. The Ssu72 phosphatase mediates the RNA polymerase II initiation-elongation transition. PMID: 25339178
    2. Ssu72 phosphatase-dependent erasure of phospho-Ser7 marks on the RNA polymerase II C-terminal domain is essential for viability and transcription termination. PMID: 22235117
    3. The mechanisms of reading the CTD code by two classes of protein phosphatases, plant CPLs and yeast Ssu72, that specifically dephosphorylate Ser(5) in vitro are reported. PMID: 16148005
  • 亞細胞定位:
    Nucleus.
  • 蛋白家族:
    SSU72 phosphatase family
  • 數據庫鏈接:

    KEGG: sce:YNL222W

    STRING: 4932.YNL222W



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