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Recombinant Saccharomyces cerevisiae Protein HIR1 (HIR1), partial

  • 中文名稱:
    釀酒酵母HIR1重組蛋白
  • 貨號:
    CSB-YP327136SVG
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    釀酒酵母HIR1重組蛋白
  • 貨號:
    CSB-EP327136SVG
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    釀酒酵母HIR1重組蛋白
  • 貨號:
    CSB-EP327136SVG-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    釀酒酵母HIR1重組蛋白
  • 貨號:
    CSB-BP327136SVG
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    釀酒酵母HIR1重組蛋白
  • 貨號:
    CSB-MP327136SVG
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    HIR1
  • Uniprot No.:
  • 別名:
    HIR1; YBL008W; YBL0318Protein HIR1; Histone transcription regulator 1
  • 種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度:
    Partial
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Component of the HIR complex, which cooperates with ASF1 to promote replication-independent chromatin assembly. The HIR complex is also required for the periodic repression of three of the four histone gene loci during cell cycle as well as for autogenous regulation of the HTA1-HTB1 locus by H2A and H2B. DNA-binding by the HIR complex may repress transcription by inhibiting nucleosome remodeling by the SWI/SNF complex. The HIR complex may also be required for transcriptional silencing of centromeric, telomeric and mating-type loci in the absence of CAF-1.
  • 基因功能參考文獻:
    1. inactivation of the negative regulatory system by deletion of the HIR1 gene relieved the dependence of HTA1-HTB1 transcription on the mitotic Clb1/Cb2 cyclins PMID: 21978826
    2. Restoration of silencing to CAF-1 subunit cac1 Delta cells requires both Hir1 and Asf1 PMID: 16020781
    3. overexpression of HIR1 in the Kllsm4Delta1 mutant prevented rapid cell death during aging, reduced nuclei fragmentation & increased resistance to H2O2; expression of histone genes is lowered in the mutant indicating a relationship between HIR1 & apoptosis PMID: 16169287
    4. Asf1 and HIR are important for histone deposition but each has a different effect on histone exchange PMID: 17914459
  • 亞細胞定位:
    Nucleus. Chromosome, centromere. Note=Enriched at centromeres.
  • 蛋白家族:
    WD repeat HIR1 family
  • 數據庫鏈接:

    KEGG: sce:YBL008W

    STRING: 4932.YBL008W



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