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Recombinant Saccharomyces cerevisiae Phosphatidylinositol 3-kinase VPS34 (VPS34), partial

  • 中文名稱:
    釀酒酵母VPS34重組蛋白
  • 貨號:
    CSB-YP334903SVG
  • 規(guī)格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    釀酒酵母VPS34重組蛋白
  • 貨號:
    CSB-EP334903SVG
  • 規(guī)格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    釀酒酵母VPS34重組蛋白
  • 貨號:
    CSB-EP334903SVG-B
  • 規(guī)格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    釀酒酵母VPS34重組蛋白
  • 貨號:
    CSB-BP334903SVG
  • 規(guī)格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    釀酒酵母VPS34重組蛋白
  • 貨號:
    CSB-MP334903SVG
  • 規(guī)格:
  • 來源:
    Mammalian cell
  • 其他:

產(chǎn)品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    VPS34
  • Uniprot No.:
  • 別名:
    VPS34; END12; PEP15; VPL7; VPT29; YLR240W; L9672.10; Phosphatidylinositol 3-kinase VPS34; PI3-kinase VPS34; PI3K VPS34; PtdIns-3-kinase VPS34; EC 2.7.1.137; Carboxypeptidase Y-deficient protein 15; Vacuolar protein sorting-associated protein 34; Vacuolar protein-targeting protein 29
  • 種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度:
    Partial
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產(chǎn)品評價

相關(guān)產(chǎn)品

靶點詳情

  • 功能:
    Phosphatidylinositol 3-kinase required for cytoplasm to vacuole transport (Cvt) and autophagy as a part of the autophagy-specific VPS34 PI3-kinase complex I. This complex is essential to recruit the ATG8-phosphatidylinositol conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal structure. Also involved in endosome-to-Golgi retrograde transport as part of the VPS34 PI3-kinase complex II. This second complex is required for the endosome-to-Golgi retrieval of PEP1 and KEX2, and the recruitment of VPS5 and VPS7, two components of the retromer complex, to endosomal membranes (probably through the synthesis of a specific pool of phosphatidylinositol 3-phosphate recruiting the retromer to the endosomes). Its activation by VPS15 may lead to the phosphorylation of phosphatidylinositol in the sorting compartment membrane. Finally, it might also be involved in ethanol tolerance and cell wall integrity.
  • 基因功能參考文獻:
    1. Data indicate that class Ia Phosphatidylinositol 3-Kinase p85alpha germ line mutations associated with SHORT syndrome did not increase PI3K activity in the yeast model. PMID: 28143957
    2. mutants lacking Vps34 display multiple phenotypes indicating impaired transcription elongation. PMID: 23335340
    3. VPS34 plays a critical role in the decline of extracellular FBPase in response to glucose. PMID: 22833678
    4. Kes1-mediated regulation of the autophagy/cytoplasm to the vacuole trafficking pathway is prevented by increasing expression of the PI3K Vps34 PMID: 20729555
    5. Gpa1 is present at endosomes, where it interacts directly with both Vps34 and Vps15 and stimulates increased production of phosphatidylinositol 3-phosphate. PMID: 16839886
    6. Study shows that the lipid-kinase activity of Vps34 is required for autophagy, implying an essential role of its product PtdIns(3)P. PMID: 18533003

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  • 亞細胞定位:
    Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein.
  • 蛋白家族:
    PI3/PI4-kinase family
  • 數(shù)據(jù)庫鏈接:

    KEGG: sce:YLR240W

    STRING: 4932.YLR240W



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