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Recombinant Saccharomyces cerevisiae Peptidyl-prolyl cis-trans isomerase ESS1 (ESS1)

  • 中文名稱:
    釀酒酵母ESS1重組蛋白
  • 貨號:
    CSB-YP328345SVG
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    釀酒酵母ESS1重組蛋白
  • 貨號:
    CSB-EP328345SVG
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    釀酒酵母ESS1重組蛋白
  • 貨號:
    CSB-EP328345SVG-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    釀酒酵母ESS1重組蛋白
  • 貨號:
    CSB-BP328345SVG
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    釀酒酵母ESS1重組蛋白
  • 貨號:
    CSB-MP328345SVG
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    ESS1
  • Uniprot No.:
  • 別名:
    ESS1; PIN1; PTF1; YJR017C; J1452Peptidyl-prolyl cis-trans isomerase ESS1; PPIase ESS1; EC 5.2.1.8; Parvulin ESS1; Processing/termination factor 1
  • 種屬:
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-170
  • 氨基酸序列
    MPSDVASRTG LPTPWTVRYS KSKKREYFFN PETKHSQWEE PEGTNKDQLH KHLRDHPVRV RCLHILIKHK DSRRPASHRS ENITISKQDA TDELKTLITR LDDDSKTNSF EALAKERSDC SSYKRGGDLG WFGRGEMQPS FEDAAFQLKV GEVSDIVESG SGVHVIKRVG
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Essential PPIase specific for phosphoserine and phosphothreonine N-terminal to the proline residue. Required for efficient pre-mRNA 3'-end processing and transcription termination, probably by inducing conformational changes by proline-directed isomerization in the C-terminal domain (CTD) of RPB1, thereby altering cofactor binding with the RNA polymerase II transcription complex. Also targets the SIN3-RPD3 histone deacetylase complex (HDAC).
  • 基因功能參考文獻:
    1. In this way, Ess1 plays an integral role in writing (and reading) the so-called CTD code to promote production of mature RNA pol II transcripts including non-coding RNAs and mRNAs. PMID: 24530645
    2. ESS1 associates with the phospho-Ser5 form of the RNA polymerase II (pol II) in vivo, is present along the entire length of coding genes, and is critical for regulating the phosphorylation of Ser7 within the pol II carboxy-terminal domain. PMID: 22778132
    3. Ess1-directed conformational changes promote dephosphorylation of Ser5 to stimulate preinitiation complex formation and, later, to inhibit elongation PMID: 15166139
    4. a level of fewer than 400 molecules per cell is sufficient for growth vs. approximately 200,000 molecules per WT cell PMID: 15728580
    5. Here we report that the exonic splicing silencer (ESS1) that represses splicing of PTPRC (encoding CD45) exon 4 does not function by the typical mechanism of inhibiting binding of U1 or U2 small nuclear ribonucleoproteins (snRNPs) to the splice sites. PMID: 16998487
    6. analysis of an alternative catalytic mechanism for Pin1 PMID: 17113106
    7. The Ess1 peptidyl prolyl isomerase functionally interacts with the transcription initiation factor TFIIB and with the Ssu72 C-terminal domain phosphatase and Pta1 components of the CPF 3'-end processing complex. PMID: 19332564
    8. Study suggests that the Pin1 activity is often translated into a fate-determining ubiquitylation switch, and that Pin1 may affect the degree of substrate ubiquitylation in other pathways as well. PMID: 19597489
    9. The prolyl isomerase Ess1 is required for Nrd1-dependent termination of noncoding RNAs. PMID: 19854134

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  • 亞細胞定位:
    Cytoplasm. Nucleus.
  • 蛋白家族:
    PpiC/parvulin rotamase family
  • 數據庫鏈接:

    KEGG: sce:YJR017C

    STRING: 4932.YJR017C



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