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  4. Recombinant Saccharomyces cerevisiae Non-histone chromosomal protein 6B (NHP6B)

Recombinant Saccharomyces cerevisiae Non-histone chromosomal protein 6B (NHP6B)

  • 中文名稱：
    釀酒酵母NHP6B重組蛋白
  • 貨號：
    CSB-YP320890SVG
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母NHP6B重組蛋白
  • 貨號：
    CSB-EP320890SVG
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母NHP6B重組蛋白
  • 貨號：
    CSB-EP320890SVG-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母NHP6B重組蛋白
  • 貨號：
    CSB-BP320890SVG
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母NHP6B重組蛋白
  • 貨號：
    CSB-MP320890SVG
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    NHP6B
  • Uniprot No.：
  • 別名：
    NHP6B; NHPB; YBR089C-A; YBR090BC; Non-histone chromosomal protein 6B
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-99
  • 氨基酸序列
    MAATKEAKQP KEPKKRTTRR KKDPNAPKRG LSAYMFFANE NRDIVRSENP DVTFGQVGRI LGERWKALTA EEKQPYESKA QADKKRYESE KELYNATRA
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    DNA-binding protein that induces severe bending of DNA. Required for DNA-binding by the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. Also augments the fidelity of transcription by RNA polymerase III independently of any role in the FACT complex. Required for transcriptional initiation fidelity of some but not all tRNA genes. Seems to be functionally redundant with NHP6A.
  • 基因功能參考文獻：
    1. Nhp6 proteins, the HMGB1 homologues, control histone gene expression by affecting nucleosome stability at regulative regions of the histone clusters. In addition, we observe that histone gene overexpression in the nhp6ab mutant is accompanied by downregulated translation, which in turn is responsible for the histone decrease phenotype. PMID: 28025045
    2. This review describes the genetic and biochemical experiments that indicate Nhp6 functions in promoting RNA pol III transcription, in formation of preinitiation complexes at promoters, and in facilitating the activity of chromatin modifying complexes. PMID: 20123079
    3. Nhp6A/B loss leads to increased genomic instability, hypersensitivity to DNA-damaging agents, and shortened yeast cell life span that is associated with elevated levels of extrachromosomal rDNA circles. PMID: 15649368
    4. Combining either a nhp6ab or a gcn5 mutation with mot1, ccr4, not4, or not5 mutations results in lethality. PMID: 16272410
    5. Nhp6 is required for transcriptional initiation fidelity of some but not all tRNA genes, in vivo PMID: 16407207
    6. NHP6B is required for transcription of a subset of tRNA genes and heterochromatin barrier function. PMID: 17178828

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  • 亞細胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    NHP6 family
  • 數據庫鏈接：


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